docs: updated CHANGELOG #1218
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failed
Mar 1, 2025 in 0s
1 tests run, 0 passed, 0 skipped, 1 failed.
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nf.test-dataset_simpleaf_aligner
Assertion failed:
11 of 16 assertions failed
Raw output
Nextflow stdout:
ERROR ~ Error executing process > 'NFCORE_SCRNASEQ:SCRNASEQ:SIMPLEAF:SIMPLEAF_QUANT (Sample_Y)'
Caused by:
Process `NFCORE_SCRNASEQ:SCRNASEQ:SIMPLEAF:SIMPLEAF_QUANT (Sample_Y)` terminated with an error exit status (1)
Command executed:
# export required var
export ALEVIN_FRY_HOME=.
# prep simpleaf
simpleaf set-paths
# run simpleaf quant
simpleaf quant \
--t2g-map t2g_3col.tsv \
--chemistry 10xv2 \
--index index \
--reads1 Sample_Y_S1_L001_R1_001.fastq.gz,Sample_Y_S1_L002_R1_001.fastq.gz \
--reads2 Sample_Y_S1_L001_R2_001.fastq.gz,Sample_Y_S1_L002_R2_001.fastq.gz \
--resolution cr-like \
--output simpleaf_quant \
--threads 2 \
--anndata-out \
--unfiltered-pl 10x_V2_barcode_whitelist.txt.gz \
cat <<-END_VERSIONS > versions.yml
"NFCORE_SCRNASEQ:SCRNASEQ:SIMPLEAF:SIMPLEAF_QUANT":
alevin-fry: $(alevin-fry --version | sed -e "s/alevin-fry //g")
piscem: $(piscem --version | sed -e "s/piscem //g")
salmon: $(salmon --version | sed -e "s/salmon //g")
simpleaf: $(simpleaf --version | sed -e "s/simpleaf //g")
END_VERSIONS
Command exit status:
1
Command output:
found `piscem` in the PATH at /usr/local/bin/piscem
found `salmon` in the PATH at /usr/local/bin/salmon
found `alevin-fry` in the PATH at /usr/local/bin/alevin-fry
[2m2025-03-01T01:54:56.659805Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m Could not find macs3 executable, peak calling cannot be peformed by simpleaf
[2m2025-03-01T01:54:56.737143Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m found file compressed using Gzip
[2m2025-03-01T01:54:56.737276Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m piscem map-sc cmd : /usr/local/bin/piscem map-sc --index index/piscem_idx --threads 2 -o simpleaf_quant/af_map --max-ec-card 4096 --skipping-strategy permissive --max-hit-occ 256 --max-hit-occ-recover 1024 --max-read-occ 2500 -1 Sample_Y_S1_L001_R1_001.fastq.gz,Sample_Y_S1_L002_R1_001.fastq.gz -2 Sample_Y_S1_L001_R2_001.fastq.gz,Sample_Y_S1_L002_R2_001.fastq.gz --geometry chromium_v2
[2m2025-03-01T01:54:58.738656Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:58.738690Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m alevin-fry generate-permit-list cmd : /usr/local/bin/alevin-fry generate-permit-list -i simpleaf_quant/af_map -d fw --unfiltered-pl 10x_V2_barcode_whitelist.txt.gz --min-reads 10 -o simpleaf_quant/af_quant
[2m2025-03-01T01:54:58.997685Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:58.997766Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m alevin-fry collate cmd : /usr/local/bin/alevin-fry collate -i simpleaf_quant/af_quant -r simpleaf_quant/af_map -t 2
[2m2025-03-01T01:54:59.006392Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:59.006608Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m cmd : "/usr/local/bin/alevin-fry" "quant" "-i" "simpleaf_quant/af_quant" "-o" "simpleaf_quant/af_quant" "-t" "2" "-m" "t2g_3col.tsv" "-r" "cr-like"
[2m2025-03-01T01:54:59.014640Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:59.014892Z[0m [33m WARN[0m [2maf_anndata[0m[2m:[0m Could not find the `gene_id_to_name` file, so only gene IDs and not symbols will be present in `var`
[2m2025-03-01T01:54:59.016695Z[0m [32m INFO[0m [2maf_anndata[0m[2m:[0m USA mode : true
Command error:
found `piscem` in the PATH at /usr/local/bin/piscem
found `salmon` in the PATH at /usr/local/bin/salmon
found `alevin-fry` in the PATH at /usr/local/bin/alevin-fry
[2m2025-03-01T01:54:56.659805Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m Could not find macs3 executable, peak calling cannot be peformed by simpleaf
[2m2025-03-01T01:54:56.737143Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m found file compressed using Gzip
[2m2025-03-01T01:54:56.737276Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m piscem map-sc cmd : /usr/local/bin/piscem map-sc --index index/piscem_idx --threads 2 -o simpleaf_quant/af_map --max-ec-card 4096 --skipping-strategy permissive --max-hit-occ 256 --max-hit-occ-recover 1024 --max-read-occ 2500 -1 Sample_Y_S1_L001_R1_001.fastq.gz,Sample_Y_S1_L002_R1_001.fastq.gz -2 Sample_Y_S1_L001_R2_001.fastq.gz,Sample_Y_S1_L002_R2_001.fastq.gz --geometry chromium_v2
[2m2025-03-01T01:54:58.738656Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:58.738690Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m alevin-fry generate-permit-list cmd : /usr/local/bin/alevin-fry generate-permit-list -i simpleaf_quant/af_map -d fw --unfiltered-pl 10x_V2_barcode_whitelist.txt.gz --min-reads 10 -o simpleaf_quant/af_quant
[2m2025-03-01T01:54:58.997685Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:58.997766Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m alevin-fry collate cmd : /usr/local/bin/alevin-fry collate -i simpleaf_quant/af_quant -r simpleaf_quant/af_map -t 2
[2m2025-03-01T01:54:59.006392Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:59.006608Z[0m [32m INFO[0m [2msimpleaf::simpleaf_commands::quant[0m[2m:[0m cmd : "/usr/local/bin/alevin-fry" "quant" "-i" "simpleaf_quant/af_quant" "-o" "simpleaf_quant/af_quant" "-t" "2" "-m" "t2g_3col.tsv" "-r" "cr-like"
[2m2025-03-01T01:54:59.014640Z[0m [32m INFO[0m [2msimpleaf::utils::prog_utils[0m[2m:[0m command returned successfully (exit status: 0)
[2m2025-03-01T01:54:59.014892Z[0m [33m WARN[0m [2maf_anndata[0m[2m:[0m Could not find the `gene_id_to_name` file, so only gene IDs and not symbols will be present in `var`
[2m2025-03-01T01:54:59.016695Z[0m [32m INFO[0m [2maf_anndata[0m[2m:[0m USA mode : true
Error: no data: empty CSV
Work dir:
/home/runner/work/scrnaseq/scrnaseq/.nf-test/tests/bd38a769ad3f15ed40dc61fc091ff019/work/83/7b084d23aa21abb488592463d4f46b
Container:
quay.io/biocontainers/simpleaf:0.19.2--ha6fb395_0
Tip: view the complete command output by changing to the process work dir and entering the command `cat .command.out`
-- Check '/home/runner/work/scrnaseq/scrnaseq/.nf-test/tests/bd38a769ad3f15ed40dc61fc091ff019/meta/nextflow.log' file for details
ERROR ~ Pipeline failed. Please refer to troubleshooting docs: https://nf-co.re/docs/usage/troubleshooting
-- Check '/home/runner/work/scrnaseq/scrnaseq/.nf-test/tests/bd38a769ad3f15ed40dc61fc091ff019/meta/nextflow.log' file for details
Nextflow stderr:
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