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Template update for nf-core/tools version 3.0.2
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nf-core-bot committed Oct 16, 2024
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3 changes: 2 additions & 1 deletion .nf-core.yml
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Expand Up @@ -19,6 +19,7 @@ template:
name: tfactivity
org: nf-core
outdir: .
skip_features: null
skip_features:
- fastqc
version: 1.0dev
update: null
4 changes: 0 additions & 4 deletions CITATIONS.md
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## Pipeline tools

- [FastQC](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/)

> Andrews, S. (2010). FastQC: A Quality Control Tool for High Throughput Sequence Data [Online].
- [MultiQC](https://pubmed.ncbi.nlm.nih.gov/27312411/)

> Ewels P, Magnusson M, Lundin S, Käller M. MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics. 2016 Oct 1;32(19):3047-8. doi: 10.1093/bioinformatics/btw354. Epub 2016 Jun 16. PubMed PMID: 27312411; PubMed Central PMCID: PMC5039924.
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1 change: 0 additions & 1 deletion README.md
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workflows use the "tube map" design for that. See https://nf-co.re/docs/contributing/design_guidelines#examples for examples. -->
<!-- TODO nf-core: Fill in short bullet-pointed list of the default steps in the pipeline -->

1. Read QC ([`FastQC`](https://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
2. Present QC for raw reads ([`MultiQC`](http://multiqc.info/))

## Usage
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4 changes: 1 addition & 3 deletions conf/modules.config
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Expand Up @@ -18,9 +18,7 @@ process {
saveAs: { filename -> filename.equals('versions.yml') ? null : filename }
]

withName: FASTQC {
ext.args = '--quiet'
}

withName: 'MULTIQC' {
ext.args = { params.multiqc_title ? "--title \"$params.multiqc_title\"" : '' }
publishDir = [
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14 changes: 0 additions & 14 deletions docs/output.md
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Expand Up @@ -12,23 +12,9 @@ The directories listed below will be created in the results directory after the

The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes data using the following steps:

- [FastQC](#fastqc) - Raw read QC
- [MultiQC](#multiqc) - Aggregate report describing results and QC from the whole pipeline
- [Pipeline information](#pipeline-information) - Report metrics generated during the workflow execution

### FastQC

<details markdown="1">
<summary>Output files</summary>

- `fastqc/`
- `*_fastqc.html`: FastQC report containing quality metrics.
- `*_fastqc.zip`: Zip archive containing the FastQC report, tab-delimited data file and plot images.

</details>

[FastQC](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/) gives general quality metrics about your sequenced reads. It provides information about the quality score distribution across your reads, per base sequence content (%A/T/G/C), adapter contamination and overrepresented sequences. For further reading and documentation see the [FastQC help pages](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/Help/).

### MultiQC

<details markdown="1">
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5 changes: 0 additions & 5 deletions modules.json
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"https://github.com/nf-core/modules.git": {
"modules": {
"nf-core": {
"fastqc": {
"branch": "master",
"git_sha": "666652151335353eef2fcd58880bcef5bc2928e1",
"installed_by": ["modules"]
},
"multiqc": {
"branch": "master",
"git_sha": "cf17ca47590cc578dfb47db1c2a44ef86f89976d",
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5 changes: 0 additions & 5 deletions modules/nf-core/fastqc/environment.yml

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64 changes: 0 additions & 64 deletions modules/nf-core/fastqc/main.nf

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66 changes: 0 additions & 66 deletions modules/nf-core/fastqc/meta.yml

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