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Remove zellkonverter, trouble installing anaconda with basilisk
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lambdamoses committed Nov 29, 2023
1 parent fa5abb8 commit 53d622f
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3 changes: 1 addition & 2 deletions DESCRIPTION
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Expand Up @@ -95,8 +95,7 @@ Suggests:
tidyr,
uwot,
vdiffr,
vroom,
zellkonverter
vroom
VignetteBuilder:
knitr
biocViews:GeneExpression, Spatial, Transcriptomics, Visualization
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Binary file added splitseq.rds
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Binary file added vignettes/10x_nuclei.rds
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Binary file added vignettes/10xatac.rds
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Binary file added vignettes/10xcrispr.rds
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Binary file added vignettes/10xmultiome.rds
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Binary file added vignettes/clicktags.rds
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Binary file added vignettes/seqfish_em3.Rds
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9 changes: 2 additions & 7 deletions vignettes/vig10_10x_nuclei.Rmd
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Expand Up @@ -63,17 +63,12 @@ library(SpatialFeatureExperiment)
library(scater)
library(scuttle)
library(Voyager)
library(zellkonverter)
library(ggplot2)
theme_set(theme_bw())
```

```{r dwnld, message=FALSE, results='hide'}
download.file("https://github.com/cellatlas/cellatlas/raw/main/examples/rna-10xv3-nuclei/cellatlas_out/adata.h5ad.gz",
"adata.h5ad.gz", quiet = TRUE)
system("gunzip -f adata.h5ad.gz")
sce <- readH5AD("adata.h5ad")
assayNames(sce) <- "counts"
```{r}
sce <- readRDS("10xnuclei.rds")
```

```{r}
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9 changes: 2 additions & 7 deletions vignettes/vig11_clicktags.Rmd
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Expand Up @@ -64,17 +64,12 @@ library(SpatialFeatureExperiment)
library(scater)
library(scuttle)
library(Voyager)
library(zellkonverter)
library(ggplot2)
theme_set(theme_bw())
```

```{r dwnld, message=FALSE, results='hide'}
download.file("https://github.com/cellatlas/cellatlas/raw/main/examples/tag-clicktag/cellatlas_out/adata.h5ad.gz",
"adata.h5ad.gz", quiet = TRUE)
system("gunzip -f adata.h5ad.gz")
sce <- readH5AD("adata.h5ad")
assayNames(sce) <- "counts"
```{r}
sce <- readRDS("clicktags.rds")
```

```{r}
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8 changes: 2 additions & 6 deletions vignettes/vig12_crispr.Rmd
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Expand Up @@ -64,16 +64,12 @@ library(SpatialFeatureExperiment)
library(scater)
library(scuttle)
library(Voyager)
library(zellkonverter)
library(ggplot2)
theme_set(theme_bw())
```

```{r dwnld, message=FALSE, results='hide'}
download.file("https://github.com/cellatlas/cellatlas/raw/main/examples/crispr-10xcrispr/cellatlas_out/adata.h5ad.gz", "adata.h5ad.gz", quiet = TRUE)
system("gunzip -f adata.h5ad.gz")
sce <- readH5AD("adata.h5ad")
assayNames(sce) <- "counts"
```{r}
sce <- readRDS("10xcrispr.rds")
```

```{r}
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10 changes: 2 additions & 8 deletions vignettes/vig13_10xatac.Rmd
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Expand Up @@ -65,17 +65,11 @@ library(SpatialFeatureExperiment)
library(scater)
library(scuttle)
library(Voyager)
library(zellkonverter)
library(ggplot2)
theme_set(theme_bw())
```

```{r dwnld, message=FALSE, results='hide'}
download.file("https://github.com/cellatlas/cellatlas/raw/main/examples/atac-10xatac/cellatlas_out/adata.h5ad.gz",
"adata.h5ad.gz", quiet = TRUE)
system("gunzip -f adata.h5ad.gz")
sce <- readH5AD("adata.h5ad")
assayNames(sce) <- "counts"
```{r}
sce <- readRDS("10xatac.rds")
```

```{r}
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9 changes: 2 additions & 7 deletions vignettes/vig14_10xmultiome.Rmd
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Expand Up @@ -65,17 +65,12 @@ library(SpatialFeatureExperiment)
library(scater)
library(scuttle)
library(Voyager)
library(zellkonverter)
library(ggplot2)
theme_set(theme_bw())
```

```{r dwnld, message=FALSE, results='hide'}
download.file("https://github.com/cellatlas/cellatlas/raw/main/examples/atac-10xmultiome/cellatlas_out/adata.h5ad.gz",
"adata.h5ad.gz", quiet = TRUE)
system("gunzip -f adata.h5ad.gz")
sce <- readH5AD("adata.h5ad")
assayNames(sce) <- "counts"
```{r}
sce <- readRDS("10xmultiome.rds")
```

```{r}
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9 changes: 2 additions & 7 deletions vignettes/vig9_splitseq.Rmd
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Expand Up @@ -62,7 +62,6 @@ install.packages("anndata")
The data in this vignette is shipped with the `cellatlas` repository. The count matrix and metadata are provided in the `cellatlas/examples` folder as an [`AnnData`](https://anndata-tutorials.readthedocs.io/en/latest/getting-started.html) object. We will begin by loading the object and converting it to a `SpatialFeatureExperiment` object.

```{r setup, message=FALSE}
library(zellkonverter)
library(stringr)
library(Matrix)
library(SpatialExperiment)
Expand All @@ -72,12 +71,8 @@ library(scuttle)
library(Voyager)
```

```{r dwnld}
download.file("https://github.com/cellatlas/cellatlas/raw/main/examples/rna-splitseq/cellatlas_out/adata.h5ad.gz",
"adata.h5ad.gz", quiet = TRUE)
system("gunzip -f adata.h5ad.gz")
sce <- readH5AD("adata.h5ad")
assayNames(sce) <- "counts"
```{r}
sce <- readRDS("splitseq.rds")
```

```{r}
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