Modified the UI to include query reference

phac-nml · Dec 10, 2024 · aa4e0bd · aa4e0bd
1 parent 1efe47c
commit aa4e0bd
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Showing 17 changed files with 56 additions and 186 deletions.
diff --git a/assets/samplesheet.csv b/assets/samplesheet.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json
+sample,fastmatch_category,mlst_alleles
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json
diff --git a/assets/schema_input.json b/assets/schema_input.json
@@ -25,6 +25,14 @@
                 "pattern": "^\\S+\\.mlst(\\.subtyping)?\\.json(\\.gz)?$",
                 "errorMessage": "MLST JSON file from locidex report, cannot contain spaces and must have the extension: '.mlst.json', '.mlst.json.gz', '.mlst.subtyping.json', or 'mlst.subtyping.json.gz'"
             },
+            "fastmatch_category": {
+                "type": "string",
+                "meta": ["ref_query"],
+                "errorMessage": "Has to be either query or reference",
+                "description": "Identify whether a sample is query or reference",
+                "fa_icon": "far fa-sticky-note",
+                "enum": ["query", "reference"]
+            },
             "metadata_1": {
                 "type": "string",
                 "meta": ["metadata_1"],
@@ -82,6 +90,6 @@
                 "pattern": "^[^\\n\\t\"]+$"
             }
         },
-        "required": ["sample", "mlst_alleles"]
+        "required": ["sample","fastmatch_category", "mlst_alleles"]
     }
 }
diff --git a/nextflow.config b/nextflow.config
@@ -44,7 +44,6 @@ params {
     validate_params                  = true
 
     // FastMatch
-    fastmatch_category = null
     threshold = 1
 
     // Profile dists args

diff --git a/nextflow_schema.json b/nextflow_schema.json
@@ -45,13 +45,6 @@
             "description": "The column header names of the metadata columns.",
             "default": "",
             "properties": {
-                "fastmatch_category": {
-                    "type": "string",
-                    "errorMessage": "Has to be either query or reference",
-                    "description": "Identify whether a sample is query or reference",
-                    "fa_icon": "far fa-sticky-note",
-                    "enum": ["query", "reference"]
-                },
                 "metadata_1_header": {
                     "type": "string",
                     "default": "metadata_1",
@@ -109,8 +102,7 @@
                     "pattern": "^[^\\n\\t\"]+$"
                 }
             },
-            "fa_icon": "far fa-clipboard",
-            "required": ["fastmatch_category"]
+            "fa_icon": "far fa-clipboard"
         },
         "fastmatch": {
             "title": "FastMatch",
@@ -120,7 +112,7 @@
             "properties": {
                 "threshold": {
                     "type": "integer",
-                    "description": "The output format for distances",
+                    "description": "Comparison score threshold value",
                     "default": 1
                 }
             }

diff --git a/tests/data/samplesheets/samplesheet-addsamplename.csv b/tests/data/samplesheets/samplesheet-addsamplename.csv
@@ -1,4 +1,4 @@
-sample,sample_name,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,S 1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
-sample2,S2#,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
-sample3,S2_,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
+sample,fastmatch_category,sample_name,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,S 1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
+sample2,query,S2#,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
+sample3,reference,S2_,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
diff --git a/tests/data/samplesheets/samplesheet-hamming.csv b/tests/data/samplesheets/samplesheet-hamming.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hamming/sample1.mlst.subtyping.json,,,,,,,,
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hamming/sample2.mlst.subtyping.json,,,,,,,,
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hamming/sample3.mlst.subtyping.json,,,,,,,,
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hamming/sample1.mlst.subtyping.json,,,,,,,,
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hamming/sample2.mlst.subtyping.json,,,,,,,,
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hamming/sample3.mlst.subtyping.json,,,,,,,,
diff --git a/tests/data/samplesheets/samplesheet-hash-missing.csv b/tests/data/samplesheets/samplesheet-hash-missing.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample1.mlst.subtyping.json,,,,,,,,
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample2.mlst.subtyping.json,,,,,,,,
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample3.mlst.subtyping.json,,,,,,,,
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample1.mlst.subtyping.json,,,,,,,,
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample2.mlst.subtyping.json,,,,,,,,
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample3.mlst.subtyping.json,,,,,,,,
diff --git a/tests/data/samplesheets/samplesheet-hash-more-missing.csv b/tests/data/samplesheets/samplesheet-hash-more-missing.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample1.mlst.subtyping.json,,,,,,,,
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample2.mlst.subtyping.json,,,,,,,,
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample3-more-missing.mlst.subtyping.json,,,,,,,,
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample1.mlst.subtyping.json,,,,,,,,
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample2.mlst.subtyping.json,,,,,,,,
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/case-hash-missing/sample3-more-missing.mlst.subtyping.json,,,,,,,,
diff --git a/tests/data/samplesheets/samplesheet-little-metadata.csv b/tests/data/samplesheets/samplesheet-little-metadata.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,,,,1.4,,,,
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,,,,,,,,
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,,,,,,3.8
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,,,,1.4,,,,
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,,,,,,,,
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,,,,,,3.8
diff --git a/tests/data/samplesheets/samplesheet-mismatched-ids.csv b/tests/data/samplesheets/samplesheet-mismatched-ids.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sampleA,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
-sampleB,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
-sampleC,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sampleA,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
+sampleB,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
+sampleC,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
diff --git a/tests/data/samplesheets/samplesheet-no-metadata.csv b/tests/data/samplesheets/samplesheet-no-metadata.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,,,,,,,,
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,,,,,,,,
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,,,,,,,,
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,,,,,,,,
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,,,,,,,,
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,,,,,,,,
diff --git a/tests/data/samplesheets/samplesheet-partial-mismatched-ids.csv b/tests/data/samplesheets/samplesheet-partial-mismatched-ids.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sampleA,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
-sampleB,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sampleA,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
+sampleB,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
diff --git a/tests/data/samplesheets/samplesheet-tabs.csv b/tests/data/samplesheets/samplesheet-tabs.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,a	b,,,,,,,
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,,,,a	b,,,,
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,,,,,,,,a	b
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,a	b,,,,,,,
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,,,,a	b,,,,
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,,,,,,,,a	b
diff --git a/tests/data/samplesheets/samplesheet1.csv b/tests/data/samplesheets/samplesheet1.csv
@@ -1,4 +1,4 @@
-sample,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
-sample1,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
-sample2,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
-sample3,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
+sample,fastmatch_category,mlst_alleles,metadata_1,metadata_2,metadata_3,metadata_4,metadata_5,metadata_6,metadata_7,metadata_8
+sample1,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample1.mlst.json,1.1,1.2,1.3,1.4,1.5,1.6,1.7,1.8
+sample2,query,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample2.mlst.json,2.1,2.2,2.3,2.4,2.5,2.6,2.7,2.8
+sample3,reference,https://raw.githubusercontent.com/phac-nml/gasclustering/dev/tests/data/reports/sample3.mlst.json,3.1,3.2,3.3,3.4,3.5,3.6,3.7,3.8
diff --git a/tests/pipelines/main.nf.test b/tests/pipelines/main.nf.test
@@ -12,7 +12,6 @@ nextflow_pipeline {
                 outdir = "results"
 
                 pd_distm = "scaled"
-                gm_thresholds = "50,20,0"
 
                 metadata_1_header = "myheader_1"
                 metadata_2_header = "myheader_2"
@@ -59,7 +58,6 @@ nextflow_pipeline {
                 input = "$baseDir/tests/data/samplesheets/samplesheet-hamming.csv"
                 outdir = "results"
 
-                gm_thresholds = "2,1,0"
             }
         }
 
@@ -188,24 +186,6 @@ nextflow_pipeline {
         }
     }
 
-    test("Test fail pipeline if invalid delimiter set") {
-        tag "pipeline_failure_invalid_delimiter"
-
-        when {
-            params {
-                input = "$baseDir/tests/data/samplesheets/samplesheet-hamming.csv"
-                outdir = "results"
-
-                gm_delimiter = ';'
-            }
-        }
-
-        then {
-            assert workflow.failed
-            assert workflow.stderr.contains('* --gm_delimiter: string [;] does not match pattern ^[A-Fa-f0-9\\._-]+$ (;)')
-        }
-    }
-
     test("Full pipeline with no metadata") {
         tag "pipeline_no_metadata"
 

diff --git a/tests/pipelines/main_gm_thresholds.nf.test b/tests/pipelines/main_gm_thresholds.nf.test
@@ -3,100 +3,6 @@ nextflow_pipeline {
     name "Integration Tests of adjusting gm_thresholds parameter for clustering"
     script "main.nf"
 
-    test("Test fail pipeline if null threshold set") {
-        tag "pipeline_failure_null_threshold"
-
-        when {
-            params {
-                input = "$baseDir/tests/data/samplesheets/samplesheet-hamming.csv"
-                outdir = "results"
-
-                gm_thresholds = null
-            }
-        }
-
-        then {
-            assert workflow.failed
-            assert workflow.stdout.contains("ERROR ~ --gm_thresholds null: Cannot pass null or empty string")
-        }
-    }
-
-    test("Test fail pipeline if empty threshold set") {
-        tag "pipeline_failure_no_threshold"
-
-        when {
-            params {
-                input = "$baseDir/tests/data/samplesheets/samplesheet-hamming.csv"
-                outdir = "results"
-
-                gm_thresholds = ""
-            }
-        }
-
-        then {
-            assert workflow.failed
-            assert workflow.stdout.contains("ERROR ~ --gm_thresholds : Cannot pass null or empty string")
-        }
-    }
-
-    test("Test fail pipeline if negative threshold set") {
-        tag "pipeline_failure_negative_threshold"
-
-        when {
-            params {
-                input = "$baseDir/tests/data/samplesheets/samplesheet-hamming.csv"
-                outdir = "results"
-
-                gm_thresholds = "-1"
-            }
-        }
-
-        then {
-            assert workflow.failed
-            assert workflow.stderr.contains('* --gm_thresholds: string [-1] does not match pattern ^(\\d+(\\.\\d+)?,)*\\d+(\\.\\d+)?$ (-1)')
-        }
-    }
-
-    test("Test fail pipeline if mismatch between thresholds and scaled distm") {
-        tag "pipeline_failure_threshold_scaled"
-
-        when {
-            params {
-                input = "$baseDir/tests/data/samplesheets/samplesheet-hamming.csv"
-                outdir = "results"
-
-                gm_thresholds = "200,50"
-                pd_distm = "scaled"
-            }
-        }
-
-        then {
-            assert workflow.failed
-            assert workflow.stdout.contains("ERROR ~ '--pd_distm scaled' is set, but '--gm_thresholds 200,50' contains thresholds outside of range [0, 100]."
-                                            + " Please either set '--pd_distm hamming' or adjust the threshold values.")
-        }
-    }
-
-    test("Test fail pipeline if mismatch between thresholds and hamming distm") {
-        tag "pipeline_failure_threshold_hamming"
-
-        when {
-            params {
-                input = "$baseDir/tests/data/samplesheets/samplesheet-hamming.csv"
-                outdir = "results"
-
-                gm_thresholds = "2,0.5"
-                pd_distm = "hamming"
-            }
-        }
-
-        then {
-            assert workflow.failed
-            assert workflow.stdout.contains("ERROR ~ '--pd_distm hamming' is set, but '--gm_thresholds 2,0.5' contains fractions."
-                                            + " Please either set '--pd_distm scaled' or remove fractions from distance thresholds.")
-        }
-    }
-
     test("Test pipeline with single threshold set to 0") {
         tag "pipeline_thresh_0"
 

diff --git a/workflows/fastmatchirida.nf b/workflows/fastmatchirida.nf
@@ -127,22 +127,7 @@ workflow FASTMATCH {
         exit 1, "--pd_columns ${params.pd_columns}: Does not exist but was passed to the pipeline. Exiting now."
     }
 
-    if(params.gm_thresholds == null || params.gm_thresholds == ""){
-        exit 1, "--gm_thresholds ${params.gm_thresholds}: Cannot pass null or empty string"
-    }
-
-    gm_thresholds_list = params.gm_thresholds.toString().split(',')
-    if (params.pd_distm == 'hamming') {
-        if (gm_thresholds_list.any { it != null && it.contains('.') }) {
-            exit 1, ("'--pd_distm ${params.pd_distm}' is set, but '--gm_thresholds ${params.gm_thresholds}' contains fractions."
-                    + " Please either set '--pd_distm scaled' or remove fractions from distance thresholds.")
-        }
-    } else if (params.pd_distm == 'scaled') {
-        if (gm_thresholds_list.any { it != null && (it as Float < 0.0 || it as Float > 100.0) }) {
-            exit 1, ("'--pd_distm ${params.pd_distm}' is set, but '--gm_thresholds ${params.gm_thresholds}' contains thresholds outside of range [0, 100]."
-                    + " Please either set '--pd_distm hamming' or adjust the threshold values.")
-        }
-    } else {
+    if ((params.pd_distm != 'hamming') & (params.pd_distm != 'scaled')) {
         exit 1, "'--pd_distm ${params.pd_distm}' is an invalid value. Please set to either 'hamming' or 'scaled'."
     }