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update test and README.md
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ekushele committed Jan 20, 2022
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4 changes: 2 additions & 2 deletions README.md
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Expand Up @@ -31,7 +31,7 @@ On release, automated continuous integration tests run the pipeline on a [full-s

## Pipeline Summary

1. Basecalling and/or demultiplexing ([`Guppy`](https://nanoporetech.com/nanopore-sequencing-data-analysis) or [`qcat`](https://github.com/nanoporetech/qcat); *optional*)
1. Basecalling and/or demultiplexing ([`Guppy`](https://nanoporetech.com/nanopore-sequencing-data-analysis), [`demux_fast5`](https://github.com/nanoporetech/ont_fast5_api#demux_fast5) or [`qcat`](https://github.com/nanoporetech/qcat); *optional*)
2. Sequencing QC ([`pycoQC`](https://github.com/a-slide/pycoQC), [`NanoPlot`](https://github.com/wdecoster/NanoPlot))
3. Raw read DNA cleaning ([NanoLyse](https://github.com/wdecoster/nanolyse); *optional*)
4. Raw read QC ([`NanoPlot`](https://github.com/wdecoster/NanoPlot), [`FastQC`](http://www.bioinformatics.babraham.ac.uk/projects/fastqc/))
Expand Down Expand Up @@ -88,7 +88,7 @@ An example input samplesheet for performing both basecalling and demultiplexing

nf-core/nanoseq was originally written by [Chelsea Sawyer](https://github.com/csawye01) and [Harshil Patel](https://github.com/drpatelh) from [The Bioinformatics & Biostatistics Group](https://www.crick.ac.uk/research/science-technology-platforms/bioinformatics-and-biostatistics/) for use at [The Francis Crick Institute](https://www.crick.ac.uk/), London. Other primary contributors include [Laura Wratten](https://github.com/lwratten), [Ying Chen](https://github.com/cying111), [Yuk Kei Wan](https://github.com/yuukiiwa) and [Jonathan Goeke](https://github.com/jonathangoeke) from the [Genome Institute of Singapore](https://www.a-star.edu.sg/gis), [Johannes Alneberg](https://github.com/alneberg) and [Franziska Bonath](https://github.com/FranBonath) from [SciLifeLab](https://www.scilifelab.se/), Sweden.

Many thanks to others who have helped out along the way too, including (but not limited to): [@crickbabs](https://github.com/crickbabs), [@AnnaSyme](https://github.com/AnnaSyme).
Many thanks to others who have helped out along the way too, including (but not limited to): [@crickbabs](https://github.com/crickbabs), [@AnnaSyme](https://github.com/AnnaSyme),[@ekushele](https://github.com/ekushele).

## Contributions and Support

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27 changes: 14 additions & 13 deletions conf/test.config
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Expand Up @@ -15,19 +15,20 @@ params {
config_profile_description = 'Minimal test dataset to check pipeline function'

// Limit resources so that this can run on GitHub Actions
max_cpus = 2
max_memory = 6.GB
max_time = 12.h

// Input data to perform both basecalling and demultiplexing
input = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/samplesheet_bc_dx.csv'
protocol = 'cDNA'
flowcell = 'FLO-MIN106'
kit = 'SQK-DCS109'
barcode_kit = 'EXP-NBD103'
run_nanolyse = true
skip_quantification = true

max_cpus = 2
max_memory = 6.GB
max_time = 12.h

// Input data to per form both basecalling and demultiplexing
input = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/samplesheet_bc_dx.csv'
protocol = 'cDNA'
flowcell = 'FLO-MIN106'
kit = 'SQK-DCS109'
barcode_kit = 'EXP-NBD103'
output_demultiplex_fast5 = true
run_nanolyse = true
skip_quantification = true

// This variable is just for reference and isnt actually required for the tests
// Files are downloaded and staged using the "GetTestData" process
input_path = 'https://raw.githubusercontent.com/nf-core/test-datasets/nanoseq/fast5/barcoded/'
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