Skip to content

Commit

Permalink
fix typo (#19)
Browse files Browse the repository at this point in the history
Co-authored-by: Kevin Dalton <[email protected]>
  • Loading branch information
kmdalton and Kevin Dalton authored Aug 1, 2024
1 parent d4f49a4 commit e2d9842
Showing 1 changed file with 1 addition and 1 deletion.
2 changes: 1 addition & 1 deletion _posts/2024-07-31-multivariate-wilson.md
Original file line number Diff line number Diff line change
Expand Up @@ -36,7 +36,7 @@ Hekstra et al. (1) derive a mathematical formalism for comparative crystallograp
In every case, the structured prior is able to increase the amount of signal observed in the data.

### Time-resolved Diffraction of an Enzyme, DJ-1
Zielinski and Dolamore et al. (2) applied the Multivariate Wilson prior to DJ-1, an enzyme involved in Parkinson's disease. In this study, Lois Pollack's [team](https://pollack.research.engineering.cornell.edu/) from Cornell University developed sample mixers which allowed DJ-1 to be rapidly mixed with a substrate, methylglyoxal. Mark Wilson's [group](https://redoxbiologycenter.unl.edu/markwilsonphd) from University of Nebraska Lincoln grew the DJ-1 crystals which were recorded in in a time-resolved fashion at [BioCARS](https://biocars.uchicago.edu/), a polychromatic beamline at the Advanced Photon Source. This configuration allowed the collaborators to observe the conversion of toxic methylgloxal into non-toxic lactate. The exceptional clarity of the time-resolved difference electron density maps produced by Careless enabled new insights into the catalytic mechanism of DJ-1 including an explanation of the enantio-purity of the products.
Zielinski and Dolamore et al. (2) applied the Multivariate Wilson prior to DJ-1, an enzyme involved in Parkinson's disease. In this study, Lois Pollack's [team](https://pollack.research.engineering.cornell.edu/) from Cornell University developed sample mixers which allowed DJ-1 to be rapidly mixed with a substrate, methylglyoxal. Mark Wilson's [group](https://redoxbiologycenter.unl.edu/markwilsonphd) from University of Nebraska Lincoln grew the DJ-1 crystals which were recorded in a time-resolved fashion at [BioCARS](https://biocars.uchicago.edu/), a polychromatic beamline at the Advanced Photon Source. This configuration allowed the collaborators to observe the conversion of toxic methylgloxal into non-toxic lactate. The exceptional clarity of the time-resolved difference electron density maps produced by Careless enabled new insights into the catalytic mechanism of DJ-1 including an explanation of the enantio-purity of the products.

![Stereo chemistry of DJ-1 Lactoyl-cysteine intermediate](/assets/posts/2024-07-31-multivariate-wilson/lc_intermediate.png){: image}
*The mechanism of enantioselective hydrolysis in DJ-1 catalysis as explained by [Zielinski and Dolamore et al.](https://doi.org/10.1101/2024.07.19.604369). Water can only access the Lactoylcysteine intermediate from the solvent-exposed face of the active site. [(CC-BY-NC-ND license)](https://creativecommons.org/licenses/by-nc-nd/4.0/)*{: caption}
Expand Down

0 comments on commit e2d9842

Please sign in to comment.