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Help with grouping of cells after integration #9393

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hparaiso opened this issue Oct 11, 2024 · 0 comments
Open

Help with grouping of cells after integration #9393

hparaiso opened this issue Oct 11, 2024 · 0 comments

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@hparaiso
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Hi, what is the best way to group cells by condition after integrating? I currently have two treatment groups which undergo standard QC processing (subset based on number of RNA reads and mitochondria levels) before I integrate them. After the integration process, I am having trouble deciphering which cells belong to the specific treatment group. I was using the object$groups function to group the cells, but I believe something is wrong with my grouping since there does not seem to be any difference in the cell numbers in the clusters. Thank you for the help!

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