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High-level interface for analyzing single-molecule FRET data

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Single molecule FRET analysis

DOI

This repository contains a Python package and several Jupyter notebooks to analyze single molecule FRET data.

If you use this software in a project resulting in a scientific publication, please cite the software.

Suggested workflow:

  • Clone repository
  • Copy Jupyter notebooks from the notebooks folder to the root (this) folder.
  • Copy your data into the data folder.
  • Open 01. Tracking notebook. Run each cell and adjust file paths and parameters as needed.
  • When finished, do the same using the 02. Filter notebook.
  • Summary plots can be created with the 03. Plots notebook.

Requirements

  • Python >= 3.7
  • Jupyter Notebook or Lab
  • sdt-python >= 17.0
  • numpy
  • scipy
  • pandas
  • matplotlib
  • pims
  • ipympl
  • OpenCV

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High-level interface for analyzing single-molecule FRET data

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