63 healthy volunteers were enrolled to test how antibiotic pre-treatment altered vaccine immunogenicity.
- 21 Control patients
- 21 patients treated with oral vancomycin
- 21 pateinets treated with oral vancomycin, ciprofloxacin and metronidazole
Antibiotics were provided for 7 days (day -3 to -9) prior to vaccination with the following vaccines:
- Pneumo 23
- Rotarix
- Tetanus toxoid
Vaccination outcome was determined by a combination of fecal and serum antibody tests at periods for 28 days. Stool was collected at Day -9 (antibiotic initiation), Day 0 (vaccine administration) and Day 7 (post-vaccination) and subjected to 16S rRNA gene amplicon sequencing. Samples were demultiplexed and preprocessed with dada2 to extract amplicon sequence variants (ASVs). ASVs were analyzed using R with heavy reliance on PhyloSeq.
Harris VC, Haak BW, Handley SA, Jiang B, Velasquez DE, Hykes BL Jr, Droit L, Berbers GAM, Kemper EM, van Leeuwen EMM, Boele van Hensbroek M, Wiersinga WJ. Effect of Antibiotic-Mediated Microbiome Modulation on Rotavirus Vaccine Immunogenicity: A Human, Randomized-Control Proof-of-Concept Trial. Cell Host Microbe. 2018 Aug 8;24(2):197-207.e4. doi: 10.1016/j.chom.2018.07.005. PMID: 30092197.
Raw data is associated with sequence archive PRJEB25634.
Scott Handley: [email protected] Vanessa Harris: [email protected]