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@cuSTEMized @immunogenomics @villani-lab

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slowkow/README.md

Hi there, I'm Kamil ๐Ÿ‘‹

I work as a biomedical researcher at Mass General Brigham, where I focus on statistical analysis for projects that involve millions of cells from hundreds of patient samples.

My research experience is in the fields of human genetics, functional genomics, and immunology. I specialize in the analysis of single-cell transcriptomics data (scRNA-seq).

In other words, I study data that tells us what cells are doing at the molecular level, and I compare the cells from different people to find out what might be happening in the context of diseases like rheumatoid arthritis, immunotherapy-related colitis, and COVID-19.

I also have research experience in human genetics, with a focus on immunology and the human leukocyte antigen (HLA) genes.

Single cell RNA-seq data

170k cells from 350 patients. Each point represents a cell. Color represents a cell's assignment to a cluster of cells, based on its similarity to other cells. Each cell is embedded in 3 dimensions based on its gene expression profile. (Make your own animation in R.)

Harmony can help to integrate many batches of high-dimensional data (R package or Python package).

A human HLA-TCR complex (6py2)

Two human proteins. The HLA protein (pink and purple subunits) is at the bottom and it is presenting a peptide (green) to the TCR protein (orange and yellow subunits) on top. The peptide is a fragment of the glutenin protein from wheat flour. This presentation mechanism is the basis for our adaptive immunity that protects us from pathogens and cancers, but it can also make us susceptible to autoimmune diseases.

Find tools and databases for analyzing HLA and VDJ genes at slowkow/awesome-vdj.

Pinned Loading

  1. hlabud hlabud Public

    ๐Ÿถ hlabud: HLA genotype analysis in R

    TeX 16

  2. cellguide cellguide Public

    ๐Ÿงญ Navigate single-cell RNA-seq datasets in your web browser.

    JavaScript 26 4

  3. ggrepel ggrepel Public

    ๐Ÿ“ Repel overlapping text labels away from each other in your ggplot2 figures.

    R 1.2k 96

  4. harmonypy harmonypy Public

    ๐ŸŽผ Integrate multiple high-dimensional datasets with fuzzy k-means and locally linear adjustments.

    Python 200 23

  5. snakefiles snakefiles Public

    ๐Ÿ Snakefiles for common RNA-seq data analysis workflows (STAR and Kallisto).

    Python 89 36

  6. tftargets tftargets Public

    ๐ŸŽฏ Human transcription factor target genes from 6 databases in convenient R format.

    R 86 19