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bgruening authored Jun 25, 2023
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year = {2023}
}

@article{iniesta-pallares_changes_2023,
abstract = {The Doñana wetlands comprise an emblematic Mediterranean landscape protected as a UNESCO World Heritage Site. Some parts of these wetlands have been transformed into intensive rice cultivation areas, which are currently the most productive rice-growing areas in Europe. We examined the bacterial communities in these domesticated soils as they are key for plant health and productivity and have a strong influence on biochemical cycles. To identify the bacteria, we used metabarcoding analysis coupled with metabolic predictions and co-occurrence networks. This analysis was performed in the bulk and rhizosphere soils during different stages in the growing season. These soil compartments had a greater effect on the bacterial communities than the plant phenological stages. The diversity and richness of the bacterial population inhabiting the rhizosphere was much lower than that in the bulk soil, comprising taxa that were significantly more represented in this soil compartment, such as bacteria from the genus Hydrogenophaga, three genera from the order Rhizobiales, and unclassified genera from the families Desulfocapsaceae and Actinobacteria. Rhizosphere co-occurrence networks revealed a high number of negative connections, indicating unstable bacterial communities that may be highly influenced by biotic and abiotic factors. Rhizosphere networks mostly rely on two taxa belonging to the phyla Proteobacteria and Cyanobacteria, which are the predicted network hubs in this soil compartment. The bulk soil conserved high bacterial diversity and richness that was stable throughout the growth period of rice. Anaerobic bacteria from genera Marmoricola, the uncultured Gemmatimonadota bacteria SDR1034 terrestrial group, Anaerolinea, and the sulphur oxidizer, Thiobacillus were highly represented. This analysis provides valuable information for understanding bacterial diversity in the rhizosphere of rice cultivated in this region, which is critical for enhancing plant growth and productivity.},
author = {Iniesta-Pallarés, Macarena and Brenes-Álvarez, Manuel and Lasa, Ana V. and Fernández-López, Manuel and Álvarez, Consolación and Molina-Heredia, Fernando P. and Mariscal, Vicente},
doi = {10.1016/j.apsoil.2023.105013},
issn = {0929-1393},
journal = {Applied Soil Ecology},
keywords = {16S rRNA, {\textgreater}UseGalaxy.eu, Bacterial potential functionality, Bacteriome, Co-occurrence networks, Metabarcoding, Rhizosphere},
language = {en},
month = {October},
pages = {105013},
title = {Changes in rice rhizosphere and bulk soil bacterial communities in the {Doñana} wetlands at different growth stages},
url = {https://www.sciencedirect.com/science/article/pii/S0929139323002111},
urldate = {2023-06-20},
volume = {190},
year = {2023}
}

@techreport{izzo_nucleophosmin_2023,
abstract = {Background The histone methyltransferase DOT1L catalyzes methylation of H3K79 and it is highly conserved in mammals. DOT1L plays a functional role in several biological processes including cell cycle regulation, DNA repair, RNA splicing and gene expression, suggesting a complex role in chromatin organization and regulation. Such a remarkable range of functions performed by DOT1L can be the result, at least partially, of its interaction with a plethora of proteins and presence in different complexes.
Results Here, we characterized the cooperation of DOT1L with the nucleolar protein NPM1 and the impact of both proteins on peri-nucleolar heterochromatin activity. We show that i) DOT1L interacts preferentially with monomeric NPM1 in the nucleus; ii) DOT1L acts in concert with NPM1 to maintain each other’s protein homeostasis; iii) NPM1 depletion results in H3K79me2 upregulation at chromatin remodeling genes but does not affect their expression; iv) DOT1L and NPM1 preserved DNA satellite expression at perinucleolar heterochromatin via epigenetic mechanisms dependent on H3K27me3.
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