Update citations (#1286)

Co-authored-by: bgruening <[email protected]>

_bibliography/citations-eu.bib

@@ -10331,6 +10331,27 @@ @article{strateva_first_2024
  year = {2024}
 }
 
+@article{strateva_genomic_2024,
+ abstract = {Extensively drug-resistant P. aeruginosa (XDR-PA) has been highlighted as a serious public health threat. The present study aimed to explore the genomic characteristics of two Vietnamese extended-spectrum β-lactamase-9 (VEB-9)-producing XDR-PA isolates from Bulgaria in comparison to all blaVEB-9-positive strains with available genomes. The isolates designated Pae51 and Pae52 were obtained from tracheobronchial aspirates of intensive care unit (ICU) patients. Antimicrobial susceptibility testing, whole-genome sequencing, RT-qPCR, and phylogenomic analysis were performed. Pae51 and Pae52 were resistant to most antipseudomonal β-lactams including carbapenems, aminoglycosides, and fluoroquinolones but remained susceptible to colistin and cefiderocol. Numerous resistance determinants were detected: blaVEB-9, blaPDC-3, blaOXA-10, blaOXA-50, aac(6′)-II, ant(2″)-Ia, ant(3″)-IIa, aph(3′)-IIb, cprP, catB7, dfrB2, sul1, fosA, and tet(A). Both isolates carried complex integrons with blaVEB-9 and tet(A) embedded next to the conservative 3′ end sequences. A variety of virulence factors were also identified, including the type III secretion system exotoxin U. Pae51 and Pae52 differed by only four SNPs and belonged to the high-risk clone ST357. To our knowledge, this is the first report of blaVEB-9-positive XDR-PA isolates in Bulgaria presenting a detailed genomic analysis. The development of novel antimicrobial strategies for such pathogens should be an essential part of infection control stewardship practices in ICU wards.},
+ author = {Strateva, Tanya and Stratev, Alexander and Peykov, Slavil},
+ copyright = {http://creativecommons.org/licenses/by/3.0/},
+ doi = {10.3390/pathogens13090719},
+ issn = {2076-0817},
+ journal = {Pathogens},
+ keywords = {\textit{Pseudomonas aeruginosa}, {\textgreater}UseGalaxy.eu, VEB-9 extended-spectrum β-lactamase, carbapenem resistance, exotoxin U, extensive drug resistance, high-risk clone ST357, phylogenomic analysis, whole-genome sequencing},
+ language = {en},
+ month = {September},
+ note = {Number: 9
+Publisher: Multidisciplinary Digital Publishing Institute},
+ number = {9},
+ pages = {719},
+ title = {Genomic {Insights} into {Vietnamese} {Extended}-{Spectrum} β-{Lactamase}-9-{Producing} {Extensively} {Drug}-{Resistant} {Pseudomonas} aeruginosa {Isolates} {Belonging} to the {High}-{Risk} {Clone} {ST357} {Obtained} from {Bulgarian} {Intensive} {Care} {Unit} {Patients}},
+ url = {https://www.mdpi.com/2076-0817/13/9/719},
+ urldate = {2024-08-29},
+ volume = {13},
+ year = {2024}
+}
+
 @article{strateva_genotypic_2023,
  abstract = {Abstract The present study aimed to explore the virulence characteristics in 221 Bulgarian nosocomial Stenotrophomonas maltophilia isolates (2011–2022) via screening for the presence of virulence genes, their mutational variability, and the corresponding enzyme activity. PCR amplification, enzymatic assays, whole-genome sequencing (WGS), and biofilm quantification on a polystyrene plate were performed. The incidence of virulence determinants was as follows: stmPr1 (encoding for the major extracellular protease StmPr1) 87.3\%, stmPr2 (minor extracellular protease StmPr2) 99.1\%, Smlt3773 locus (outer membrane esterase) 98.2\%, plcN1 (non-hemolytic phospholipase C) 99.1\%, and smf-1 (type-1 fimbriae, biofilm-related gene) 96.4\%. The 1621-bp allele of stmPr1 was most frequently found (61.1\%), followed by the combined allelic variant (17.6\%), stmPr1-negative genotype (12.7\%), and 868-bp allele (8.6\%). Protease, esterase, and lecithinase activity was observed in 95\%, 98.2\%, and 17.2\% of the isolates, respectively. The WGS-subjected isolates (n = 9) formed two groups. Five isolates possessed only the 1621-bp variant of stmPr1, higher biofilm formation ability (Optical Density at λ = 550 nm (OD550): 1.253–1.789), as well as a low number of mutations in the protease genes and smf-1. Three other isolates had only the 868-bp variant, weaker biofilm production (OD550: 0.788–1.108), and higher number of mutations within these genes. The only weak biofilm producer (OD550 = 0.177) had no stmPr1 alleles. In conclusion, the similar PCR detection rates did not allow differentiation of the isolates. In contrast, WGS permitted stmPr1 alleles-based differentiation. To the best of our knowledge, this is the first Bulgarian study presenting genotypic and phenotypic insights into virulence factors of S. maltophilia isolates.},
  author = {Strateva, Tanya and Trifonova, Angelina and Stratev, Alexander and Peykov, Slavil},