Merge pull request #10 from Laura190/main

Adjust parameters
warwickcamdu · Apr 23, 2024 · 32ec1f1 · 32ec1f1
2 parents 3b32d05 + cb09c5c
commit 32ec1f1
Showing 1 changed file with 2 additions and 2 deletions.
diff --git a/CountSpotsInCells.ijm b/CountSpotsInCells.ijm
@@ -63,10 +63,10 @@ selectImage(original);
 original=getTitle();
 Stack.setPosition(1, 1, 1);
 run("Duplicate...", "title=copy duplicate channels=1");
-run("Median...", "radius=1");
+run("Median...", "radius=2");
 roiManager("Deselect");
 roiManager("Combine");
-run("Find Maxima...", "prominence=140 output=[Point Selection]");
+run("Find Maxima...", "prominence=200 output=[Point Selection]");
 selectImage(original);
 run("Restore Selection");
 roiManager("add");