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add positive controls spreadsheet
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alexlenail committed May 30, 2023
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10 changes: 5 additions & 5 deletions _pages/crispriacelllineprimer.md
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Expand Up @@ -120,8 +120,8 @@ us to reserve GFP and mCherry for downstream experiments.
To help labs test new CRISPRi cell lines, we have deposited on Addgene our published GFP
CRISPRi reporter (Addgene: 46919). The GFP reporter allows you to generate single-cell
flow cytometry data to troubleshoot and validate CRISPRi activity and cell line production.
We have included [positive control sgGFP sequences](http://weissmanlab.ucsf.edu/links/positivecontrolsgRNAlist_V4.xlsx) to be cloned into our sgRNA expression
vector (Addgene: 60955). We have also included with this protocol a list of [positive control sgRNAs](http://weissmanlab.ucsf.edu/links/positivecontrolsgRNAlist_V4.xlsx)
We have included [positive control sgGFP sequences](/resources/positivecontrolsgRNAlist_V4.xlsx) to be cloned into our sgRNA expression
vector (Addgene: 60955). We have also included with this protocol a list of [positive control sgRNAs](/resources/positivecontrolsgRNAlist_V4.xlsx)
targeting human genes that have shown high activity across cell lines. We then use
qPCR to measure knockdown efficiency for this second type of positive control.

Expand All @@ -139,13 +139,13 @@ expression levels, we have found that we need to derive clonal cell lines and te
CRISPRa activity using a functional assay.**

To help labs test new CRISPRa cell lines, we have deposited on Addgene our published GFP
CRISPRa reporter (Addgene: Available soon). We have included [positive control sgRNA sequences](http://weissmanlab.ucsf.edu/links/positivecontrolsgRNAlist_V4.xlsx)
CRISPRa reporter (Addgene: Available soon). We have included [positive control sgRNA sequences](/resources/positivecontrolsgRNAlist_V4.xlsx)
targeting the endogenous human CXCR4 gene. Both of these positive controls can
be used to read out single cell CRISPRa activity using flow cytometry. We have also included
a [list of positive control sgRNAs](http://weissmanlab.ucsf.edu/links/positivecontrolsgRNAlist_V4.xlsx) targeting human genes that have shown high activity. We
a [list of positive control sgRNAs](/resources/positivecontrolsgRNAlist_V4.xlsx) targeting human genes that have shown high activity. We
have used qPCR to measure gene activation efficiency for this second type of positive control.
Additional protocols and reagents are linked here:
- [Positive control sgRNAs for CRISPRi and CRISPRa](http://weissmanlab.ucsf.edu/links/positivecontrolsgRNAlist_V4.xlsx)
- [Positive control sgRNAs for CRISPRi and CRISPRa](/resources/positivecontrolsgRNAlist_V4.xlsx)
- [sgRNA cloning protocol](/resources/sgRNACloningProtocol.pdf)
- [sgRNA assembly template](/resources/sgRNAAssemblyTemplate.xlsx)

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2 changes: 2 additions & 0 deletions compile_info.ipynb
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Expand Up @@ -23,6 +23,7 @@
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