Dummy node logic

config/config.yaml

@@ -56,6 +56,7 @@ algorithms:
                   b: [5, 6]
                   w: np.linspace(0,5,2)
                   d: [10]
+                  dummy_mode: ["file"] # Or "terminals", "all", "others"
 
       - name: "omicsintegrator2"
         params:
@@ -101,6 +102,8 @@ datasets:
     -
       # Labels can only contain letters, numbers, or underscores
       label: data0
+      # To run OmicsIntegrator1 with dummy nodes, add dummy.txt file to node_files
+      # or a dummy column to the node table
       node_files: ["node-prizes.txt", "sources.txt", "targets.txt"]
       # DataLoader.py can currently only load a single edge file, which is the primary network
       edge_files: ["network.txt"]

config/egfr.yaml

@@ -33,6 +33,7 @@ algorithms:
           - 0.1
         mu:
           - 0.008
+        dummy_mode: ["file"]
   -
     name: omicsintegrator2
     params:

doc/README.md

@@ -23,14 +23,22 @@ The graph algorithms below have been used (or have the potential to be used) for
 - Basha et al., ResponseNet2.0: revealing signaling and regulatory pathways connecting your proteins and genes–now with human data. _Nucleic Acids Research._ 2013. [doi:10.1093/nar/gkt532](https://dx.doi.org/10.1093%2Fnar%2Fgkt532)
 - Basha et al. ResponseNet v.3: revealing signaling and regulatory pathways connecting your proteins and genes across human tissues. _Nucleic Acids Research._ 2019. [doi:10.1093/nar/gkz421](https://dx.doi.org/10.1093%2Fnar%2Fgkz421)
 
-## Prize Collecting Steiner Forest (PCSF)
+## Prize Collecting Steiner Forest (PCSF): OmicsIntegrator1 and OmicsIntegrator2
 
 **References:**
 - Huang and Fraenkel. Integrating proteomic, transcriptional, and interactome data reveals hidden components of signaling and regulatory networks. _Science Signaling._ 2009. [doi:10.1126/scisignal.2000350](https://doi.org/10.1126/scisignal.2000350)
 - Tuncbag et al. Simultaneous reconstruction of multiple signaling pathways via the prize-collecting steiner forest problem. _Journal of Computational Biology._ 2013. [doi:10.1089/cmb.2012.0092](https://doi.org/10.1089/cmb.2012.0092)
 - Gitter et al. Sharing information to reconstruct patient-specific pathways in heterogeneous diseases.  _Pacific Symposium on Biocomputing._ 2014. [doi:10.1142/9789814583220_0005](https://doi.org/10.1142/9789814583220_0005)
 - Tuncbag et al., Network-Based Interpretation of Diverse High-Throughput Datasets through the Omics Integrator Software Package. _PLoS Computational Biology._ 2016. [doi:10.1371/journal.pcbi.1004879](https://doi.org/10.1371/journal.pcbi.1004879)
 
+One of the parameter options for OmicsIntegraor1 is `dummy_mode`.
+There are 4 dummy mode possibilities:
+ 1. `terminals`: connect the dummy node to all nodes that have been assigned prizes
+ 2. `all`:  connect the dummy node to all nodes in the interactome i.e. full set of nodes in graph
+ 3. `others`: connect the dummy node to all nodes that are not terminal nodes i.e. nodes w/o prizes
+ 4. `file`: connect the dummy node to a specific list of nodes provided in a file
+To support the `file` dummy node logic as part of OmicsIntegrator1, you can either add a separate `dummy.txt` file (and add this to the `node_files` argument in `config.yaml `) or add a `dummy` column node attribute to a file that contains `NODEID`, `prize`, `source`, etc.
+
 ## PathLinker
 
 PathLinker takes as input (1) a weighted, directed PPI network, (2) two sets of nodes: a source set (representing receptors of a pathway of interest) and a target set (representing transcriptional regulators of a pathway of interest), and (3) an integer _k_. PathLinker efficiently computes the _k_-shortest paths from any source to any target and returns the subnetwork of the top _k_ paths as the pathway reconstruction.  Later work expanded PathLinker by incorporating protein localization information to re-score tied paths, dubbed Localized PathLinker (LocPL).

input/README.md

@@ -11,8 +11,8 @@ All other columns specify additional node attributes such as prizes.
 Any nodes that are listed in a node file but are not present in one or more edges in the edge file will be removed.
 For example:
 ```
-NODEID	prize	sources	targets	active
-A	1.0		True	True
+NODEID	prize	sources	targets	active dummy
+A	1.0		True	True	True
 B	3.3	True		True
 C	2.5		True	True
 D	1.9	True	True	True

input/node-prizes.txt

@@ -1,3 +1,3 @@
-NODEID	prize	active
-A	2	true
+NODEID	prize	active	dummy
+A	2	true	true
 C	5.7	true

input/tps-egfr-prizes.txt

@@ -1,4 +1,4 @@
-NODEID	prize	sources	targets	active
+NODEID	prize	sources	targets	active	dummy
 1433Z_HUMAN	1.041379133		True	True
 41_HUMAN	3.389112802		True	True
 4ET_HUMAN	2.569973509		True	True
@@ -181,7 +181,7 @@ EF1A1_HUMAN	3.774750081		True	True
 EF1B_HUMAN	0.768939794		True	True
 EF1D_HUMAN	1.240472409		True	True
 EFNB2_HUMAN	2.222686177		True	True
-EGF_HUMAN	10	True		True
+EGF_HUMAN	10	True		True	True
 EGFR_HUMAN	6.787874699		True	True
 EGLN1_HUMAN	1.876580206		True	True
 EIF3B_HUMAN	2.048949271		True	True

spras/omicsintegrator1.py

@@ -35,19 +35,21 @@ def write_conf(filename=Path('config.txt'), w=None, b=None, d=None, mu=None, noi
         f.write('processes = 1\n')
         f.write('threads = 1\n')
 
-"""
-Omics Integrator 1 works with partially directed graphs
-- it takes in the universal input directly
 
-Expected raw input format:
-Interactor1    Interactor2   Weight    Direction
-- the expected raw input file should have node pairs in the 1st and 2nd columns, with a weight in the 3rd column and directionality in the 4th column
-- it can include repeated and bidirectional edges
-- it uses 'U' for undirected edges and 'D' for directed edges
-
-"""
 class OmicsIntegrator1(PRM):
-    required_inputs = ['prizes', 'edges']
+    """
+    Omics Integrator 1 works with partially directed graphs
+    - it takes in the universal input directly
+
+    Expected raw input format:
+    Interactor1    Interactor2   Weight    Direction
+    - the expected raw input file should have node pairs in the 1st and 2nd columns, with a weight in the 3rd column and
+    directionality in the 4th column
+    - it can include repeated and bidirectional edges
+    - it uses 'U' for undirected edges and 'D' for directed edges
+
+    """
+    required_inputs = ['prizes', 'edges', 'dummy_nodes']
 
     @staticmethod
     def generate_inputs(data, filename_map):
@@ -83,13 +85,22 @@ def generate_inputs(data, filename_map):
                         columns=['Interactor1','Interactor2','Weight','Direction'],
                         header=['protein1','protein2','weight','directionality'])
 
+        # creates the dummy_nodes file
+        if 'dummy' in data.node_table.columns:
+            dummy_df = data.node_table[data.node_table['dummy'] == True]
+            # save as list of dummy nodes
+            dummy_df.to_csv(filename_map['dummy_nodes'], index=False, columns=['NODEID'], header=None)
+        else:
+            # create empty dummy file
+            with open(filename_map['dummy_nodes'], mode='w'):
+                pass
 
     # TODO add parameter validation
     # TODO add support for knockout argument
     # TODO add reasonable default values
     # TODO document required arguments
     @staticmethod
-    def run(edges=None, prizes=None, dummy_mode=None, mu_squared=None, exclude_terms=None,
+    def run(edges=None, prizes=None, dummy_nodes=None, dummy_mode=None, mu_squared=None, exclude_terms=None,
             output_file=None, noisy_edges=None, shuffled_prizes=None, random_terminals=None,
             seed=None, w=None, b=None, d=None, mu=None, noise=None, g=None, r=None, container_framework="docker"):
         """
@@ -118,6 +129,19 @@ def run(edges=None, prizes=None, dummy_mode=None, mu_squared=None, exclude_terms
         bind_path, prize_file = prepare_volume(prizes, work_dir)
         volumes.append(bind_path)
 
+        # 4 dummy mode possibilities:
+        #   1. terminals -> connect the dummy node to all nodes that have been assigned prizes
+        #   2. all ->  connect the dummy node to all nodes in the interactome i.e. full set of nodes in graph
+        #   3. others -> connect the dummy node to all nodes that are not terminal nodes i.e. nodes w/o prizes
+        #   4. file -> connect the dummy node to a specific list of nodes provided in a file
+
+        # add dummy node file to the volume if dummy_mode is not None and it is 'file'
+        if dummy_mode == 'file':
+            if dummy_nodes is None:
+                raise ValueError("dummy_nodes file is required when dummy_mode is set to 'file'")
+            bind_path, dummy_file = prepare_volume(dummy_nodes, work_dir)
+            volumes.append(bind_path)
+
         out_dir = Path(output_file).parent
         # Omics Integrator 1 requires that the output directory exist
         out_dir.mkdir(parents=True, exist_ok=True)
@@ -139,9 +163,16 @@ def run(edges=None, prizes=None, dummy_mode=None, mu_squared=None, exclude_terms
                    '--outpath', mapped_out_dir,
                    '--outlabel', 'oi1']
 
+        # add the dummy mode argument
+        if dummy_mode is not None and dummy_mode:
+            # for custom dummy modes, add the file
+            if dummy_mode == 'file':
+                command.extend(['--dummy', dummy_file])
+            # else pass in the dummy_mode and let oi1 handle it
+            else:
+                command.extend(['--dummy', dummy_mode])
+
         # Add optional arguments
-        if dummy_mode is not None:
-            command.extend(['--dummyMode', str(dummy_mode)])
         if mu_squared is not None and mu_squared:
             command.extend(['--musquared'])
         if exclude_terms is not None and exclude_terms:

test/OmicsIntegrator1/input/oi1-dummy.txt

@@ -0,0 +1 @@
+B

test/OmicsIntegrator1/test_oi1.py

@@ -49,6 +49,7 @@ def test_oi1_all_optional(self):
         # Include all optional arguments
         OmicsIntegrator1.run(edges=TEST_DIR+'input/oi1-edges.txt',
                              prizes=TEST_DIR+'input/oi1-prizes.txt',
+                             dummy_nodes=None,
                              dummy_mode='terminals',
                              mu_squared=True,
                              exclude_terms=True,
@@ -66,6 +67,23 @@ def test_oi1_all_optional(self):
                              r=0)
         assert out_path.exists()
 
+    def test_oi1_dummy_file(self):
+        out_path = Path(OUT_FILE)
+        out_path.unlink(missing_ok=True)
+        # Include optional argument
+        OmicsIntegrator1.run(edges=TEST_DIR+'input/oi1-edges.txt',
+                             prizes=TEST_DIR+'input/oi1-prizes.txt',
+                             dummy_nodes=TEST_DIR + 'input/oi1-dummy.txt',
+                             dummy_mode='file',
+                             output_file=OUT_FILE,
+                             w=5,
+                             b=1,
+                             d=10,
+                             noise=0.333,
+                             g=0.001,
+                             r=0)
+        assert out_path.exists()
+
     def test_oi1_missing(self):
         # Test the expected error is raised when required arguments are missing
         with pytest.raises(ValueError):
@@ -81,6 +99,18 @@ def test_oi1_missing(self):
                        b=1,
                        d=10)
 
+    def test_oi1_missing_dummy(self):
+        # Test the expected error is raised when the dummy_nodes file is missing and the dummy_mode is 'file'
+        with pytest.raises(ValueError):
+            # No edges
+            OmicsIntegrator1.run(edges=TEST_DIR+'input/oi1-edges.txt',
+                                 prizes=TEST_DIR + 'input/oi1-prizes.txt',
+                                 output_file=TEST_DIR+'output/test_optimalForest.sif',
+                                 w=5,
+                                 b=1,
+                                 d=10,
+                                 dummy_mode='file')
+
     # Only run Singularity test if the binary is available on the system
     # spython is only available on Unix, but do not explicitly skip non-Unix platforms
     @pytest.mark.skipif(not shutil.which('singularity'), reason='Singularity not found on system')