CBiS

Sookmyung Women's University Center for advanced Bioinformatic&Systems medicine Intern

1. Image Normalization(Plate9)

• NC avg
• NC med
• NC sd

2. Classify STK11 mutation cell line and wildtype cell line(in Lung(LUAD) NSCLC)
   Lung → NSCLC,(SCLC,OTHER) → Check STK11 mutation

    4 Types of cell lines

• LUNG NSCLC STK11 mutant
• LUNG NSCLC STK11 wild type
• LUNG Others
• Others

Calculate fold change t-test(pvalue) 1,2 / 1,3 / 1,4

Organize CCLE
shRNA : DRIVE
sgRNA : Depmap.org

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CCLE COSMIC annotation : cell line(1267) - Major gene mutation information

DRIVE(shRNA) : cell line(398) gene(7726)

gene effect(sgRNA) : cell line(517) gene(16383)