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Merge pull request #4350 from neoformit/neoformit-patch-1
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Neoformit patch 1
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shiltemann authored Sep 20, 2023
2 parents d7bdc15 + e455b59 commit 7616b00
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Original file line number Diff line number Diff line change
Expand Up @@ -36,6 +36,7 @@ contributors:
- r1corre
- stephanierobin
- erasmusplus
- neoformit

---

Expand Down Expand Up @@ -878,7 +879,7 @@ In case of long reads, we can check sequence quality with [Nanoplot](https://git
> https://zenodo.org/api/files/ff9aa6e3-3d69-451f-9798-7ea69b475989/m64011_190830_220126.Q20.subsample.fastq.gz
> ```
>
> 3. {% tool [Nanoplot](toolshed.g2.bx.psu.edu/repos/iuc/nanoplot/nanoplot/1.28.2+galaxy1) %} with the following parameters
> 3. {% tool [Nanoplot](toolshed.g2.bx.psu.edu/repos/iuc/nanoplot/nanoplot/1.41.0+galaxy0) %} with the following parameters
> - {% icon param-files %} *"files"*: `m64011_190830_220126.Q20.subsample.fastq.gz`
> - *"Options for customizing the plots created"*
> - {% icon param-select %} *"Specify the bivariate format of the plots."*: `dot`, `kde`
Expand Down Expand Up @@ -912,7 +913,7 @@ In case of long reads, we can check sequence quality with [Nanoplot](https://git

This plot shows the distribution of fragment sizes in the file that was analyzed.
Unlike most of Illumina runs, long reads have a variable length and this will show the relative amounts of each different size of sequence fragment.
In this example, the distribution of read length is centered near 15kbp but the results can be very different depending of your experiment.
In this example, the distribution of read length is centered near 18kbp but the results can be very different depending of your experiment.

![Histogram of read lengths](../../images/quality-control/HistogramReadlength.png "Histogram of read length")

Expand Down Expand Up @@ -1087,6 +1088,6 @@ Quality control steps are similar for any type of sequencing data:
- Quality assessment with tools like:
- *Short Reads*: {% tool [FASTQE](toolshed.g2.bx.psu.edu/repos/iuc/fastqe/fastqe/0.2.6+galaxy2) %}
- *Short+Long*: {% tool [FASTQC](toolshed.g2.bx.psu.edu/repos/devteam/fastqc/fastqc/0.73+galaxy0) %}
- *Long Reads*: {% tool [Nanoplot](toolshed.g2.bx.psu.edu/repos/iuc/nanoplot/nanoplot/1.28.2+galaxy1) %}
- *Long Reads*: {% tool [Nanoplot](toolshed.g2.bx.psu.edu/repos/iuc/nanoplot/nanoplot/1.41.0+galaxy0) %}
- *Nanopore only*: {% tool [PycoQC](toolshed.g2.bx.psu.edu/repos/iuc/pycoqc/pycoqc/2.5.2+galaxy0) %}
- Trimming and filtering for **short reads** with a tool like **Cutadapt** {% icon tool %}

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