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[WIP] ProteoRE topGO enrichment analysis 20210412 #598

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10 changes: 10 additions & 0 deletions tools/proteore_topgo/.Rhistory
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library("topGO")
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that file should go and should probably be added to the .gitignore file

biocLite("topGO")
source("https://bioconductor.org/biocLite.R")
biocLite("topGO")
library("topGO")
library("topGO")
biocLite("topGO")
library("topGO")
library(topGO)
q()
5 changes: 5 additions & 0 deletions tools/proteore_topgo/.shed.yml
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categories: [Proteomics]
description: ProteoRE topGO
long_description: A tool performing enrichment analysis for Gene Ontology
name: proteore_topgo
owner: galaxyp
77 changes: 77 additions & 0 deletions tools/proteore_topgo/README.rst
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Wrapper for topGO Tool
======================

**Authors**

Alexa A and Rahnenfuhrer J (2016). topGO: Enrichment Analysis for Gene Ontology. R package version 2.30.0.

**Galaxy integration**

Lisa Perus, T.P. Lien Nguyen, Florence Combes, Yves Vandenbrouck CEA, INSERM, CNRS, Grenoble-Alpes University, BIG Institute, FR

Sandra Dérozier, Olivier Rué, Christophe Caron, Valentin Loux INRA, Paris-Saclay University, MAIAGE Unit, Migale Bioinformatics platform

This work has been partially funded through the French National Agency for Research (ANR) IFB project.

Contact [email protected] for any questions or concerns about the Galaxy implementation of this tool.

----------------------

**Galaxy component based on R package topGO.**

**Input required**

This component works with Ensembl gene ids (e.g : ENSG0000013618). You can
copy/paste these identifiers or supply a tabular file (.csv, .tsv, .txt, .tab)
where there are contained.

**Principle**

This component provides the GO terms representativity of a gene list in one ontology category (Biological Process "BP", Cellular Component "CC", Molecular Function "MF"). This representativity is evaluated in comparison to the background list of all human genes associated associated with GO terms of the chosen category (BP,CC,MF). This background is given by the R package "org.Hs.eg.db", which is a genome wide association package for **human**.

**Output**

Three kind of outputs are available : a textual output, a barplot output and
a dotplot output.

*Textual output* :
The text output lists all the GO-terms that were found significant under the specified threshold.


The different fields are as follow :

- Annotated : number of genes in org.Hs.eg.db which are annotated with the GO-term.

- Significant : number of genes belonging to your input which are annotated with the GO-term.

- Expected : show an estimate of the number of genes a node of size Annotated would have if the significant genes were to be randomly selected from the gene universe.

- pvalues : pvalue obtained after the test

- ( qvalues : additional column with adjusted pvalues )


**Tests**

topGO provides a classic fisher test for evaluating if some GO terms are over-represented in your gene list, but other options are also provided (elim, weight01,parentchild). For the merits of each option and their algorithmic descriptions, please refer to topGO manual :
https://bioconductor.org/packages/release/bioc/vignettes/topGO/inst/doc/topGO.pdf

**Multiple testing corrections**

Furthermore, the following corrections for multiple testing can also be applied :
- holm
- hochberg
- hommel
- bonferroni
- BH
- BY
- fdr

**Packages used**
- bioconductor-org.hs.eg.db v3.5.0
- bioconductor-org.mm.eg.db v3.5.0
- bioconductor-org.rn.eg.db v3.5.0
- bioconductor-annotationdbi v1.40.0
- bioconductor-go.db v3.5.0
- bioconductor-graph v1.56.0
- bioconductor-topgo v2.30.0
18 changes: 18 additions & 0 deletions tools/proteore_topgo/releases_notes.rst
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======================================================
Enrichment analysis for Gene Ontology - Releases Notes
======================================================

==================
2019-06-01 Release
==================

New
---

Improvements
------------

- doc

Fixes
-----
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Protein accession number (UniProt) Protein name Number of peptides (razor + unique) neXtProt_ID UniProt.ID GeneID MIM Ensembl
P15924 Desmoplakin 69 NX_P15924 DESP_HUMAN 1832 125647; 605676; 607450; 607655; 609638; 612908; 615821 ENSG00000096696
P02538 Keratin, type II cytoskeletal 6A 53 NX_P02538 K2C6A_HUMAN 3853 148041; 615726 ENSG00000205420
P02768 Serum albumin 44 NX_P02768 ALBU_HUMAN 213 103600; 615999; 616000 ENSG00000163631
P08779 Keratin, type I cytoskeletal 16 29 NX_P08779 K1C16_HUMAN 3868 148067; 167200; 613000 ENSG00000186832
Q02413 Desmoglein-1 24 NX_Q02413 DSG1_HUMAN 1828 125670; 148700; 615508 ENSG00000134760
P07355 Annexin A2;Putative annexin A2-like protein 22 NX_P07355 ANXA2_HUMAN 302 151740 ENSG00000182718
P14923 Junction plakoglobin 22 NX_P14923 PLAK_HUMAN 3728 173325; 601214; 611528 ENSG00000173801
P02788 Lactotransferrin 21 NX_P02788 TRFL_HUMAN 4057 150210 ENSG00000012223
Q9HC84 Mucin-5B 21 NX_Q9HC84 MUC5B_HUMAN 727897 178500; 600770 ENSG00000117983
P29508 Serpin B3 20 NX_P29508 SPB3_HUMAN 6317 600517 ENSG00000057149
P63261 Actin, cytoplasmic 2 19 NX_P63261 ACTG_HUMAN 71 102560; 604717; 614583 ENSG00000184009
Q8N1N4 Keratin, type II cytoskeletal 78 18 NX_Q8N1N4 K2C78_HUMAN 196374 611159 ENSG00000170423
Q04695 Keratin, type I cytoskeletal 17 18 NX_Q04695 K1C17_HUMAN 3872 148069; 167210; 184500 ENSG00000128422
P01876 Ig alpha-1 chain C region 16 NX_P01876 IGHA1_HUMAN NA 146900 ENSG00000211895; ENSG00000282633
Q01469 Fatty acid-binding protein 5, epidermal 15 NX_Q01469 FABP5_HUMAN 2171 605168 ENSG00000164687
P31944 Caspase-14 15 NX_P31944 CASPE_HUMAN 23581 605848; 617320 ENSG00000105141
P01833 Polymeric immunoglobulin receptor 15 NX_P01833 PIGR_HUMAN 5284 173880 ENSG00000162896
P06733 Alpha-enolase 15 NX_P06733 ENOA_HUMAN 2023 172430 ENSG00000074800
P25311 Zinc-alpha-2-glycoprotein 15 NX_P25311 ZA2G_HUMAN 563 194460 ENSG00000160862
Q15149 Plectin 15 NX_Q15149 PLEC_HUMAN 5339 131950; 226670; 601282; 612138; 613723; 616487 ENSG00000178209
P19013 Keratin, type II cytoskeletal 4 13 NX_P19013 K2C4_HUMAN NA 123940; 193900 ENSG00000170477
Q6KB66 Keratin, type II cytoskeletal 80 13 NX_Q6KB66 K2C80_HUMAN 144501 611161 ENSG00000167767
Q08188 Protein-glutamine gamma-glutamyltransferase E 12 NX_Q08188 TGM3_HUMAN 7053 600238; 617251 ENSG00000125780
P13646 Keratin, type I cytoskeletal 13 11 NX_P13646 K1C13_HUMAN 3860 148065; 615785 ENSG00000171401
Q86YZ3 Hornerin 11 NX_Q86YZ3 HORN_HUMAN 388697 616293 ENSG00000197915
P04259 Keratin, type II cytoskeletal 6B 10 NX_P04259 K2C6B_HUMAN 3854 148042; 615728 ENSG00000185479
P02545 Prelamin-A/C;Lamin-A/C 10 NX_P02545 LMNA_HUMAN 4000 115200; 150330; 151660; 159001; 176670; 181350; 212112; 248370; 275210; 605588; 610140; 613205; 616516 ENSG00000160789
P04083 Annexin A1 10 NX_P04083 ANXA1_HUMAN 301 151690 ENSG00000135046
P11021 78 kDa glucose-regulated protein 10 NX_P11021 GRP78_HUMAN 3309 138120 ENSG00000044574
P02787 Serotransferrin 9 NX_P02787 TRFE_HUMAN 7018 190000; 209300 ENSG00000091513
P04040 Catalase 9 NX_P04040 CATA_HUMAN 847 115500; 614097 ENSG00000121691
P31151 Protein S100-A7 9 NX_P31151 S10A7_HUMAN 6278 600353 ENSG00000143556
P31947 14-3-3 protein sigma 9 NX_P31947 1433S_HUMAN 2810 601290 ENSG00000175793
Q96P63 Serpin B12 9 NX_Q96P63 SPB12_HUMAN 89777 615662 ENSG00000166634
P14618 Pyruvate kinase PKM 9 NX_P14618 KPYM_HUMAN 5315 179050 ENSG00000067225
P60174 Triosephosphate isomerase 9 NX_P60174 TPIS_HUMAN 7167 190450; 615512 ENSG00000111669
Q06830 Peroxiredoxin-1 9 NX_Q06830 PRDX1_HUMAN 5052 176763 ENSG00000117450
P01040 Cystatin-A 8 NX_P01040 CYTA_HUMAN 1475 184600; 607936 ENSG00000121552
P05089 Arginase-1 8 NX_P05089 ARGI1_HUMAN 383 207800; 608313 ENSG00000118520
P01834 Ig kappa chain C region 8 NX_P01834 IGKC_HUMAN NA 147200; 614102 NA
P04406 Glyceraldehyde-3-phosphate dehydrogenase 8 NX_P04406 G3P_HUMAN 2597 138400 ENSG00000111640
P0DMV9 Heat shock 70 kDa protein 1B 8 NX_P0DMV9 HS71B_HUMAN 3303; 3304 140550; 603012 ENSG00000204388; ENSG00000224501; ENSG00000212866; ENSG00000231555; ENSG00000232804
P13639 Elongation factor 2 8 NX_P13639 EF2_HUMAN 1938 130610; 609306 ENSG00000167658
P35579 Myosin-9 8 NX_P35579 MYH9_HUMAN 4627 153640; 153650; 155100; 160775; 600208; 603622; 605249 ENSG00000100345
P68371 Tubulin beta-4B chain 8 NX_P68371 TBB4B_HUMAN 10383 602660 ENSG00000188229
Q8WVV4 Protein POF1B 8 NX_Q8WVV4 POF1B_HUMAN 79983 300603; 300604 ENSG00000124429
O75635 Serpin B7 7 NX_O75635 SPB7_HUMAN 8710 603357; 615598 ENSG00000166396
P01857 Ig gamma-1 chain C region 7 NX_P01857 IGHG1_HUMAN NA 147100; 254500 ENSG00000211896; ENSG00000277633
P61626 Lysozyme C 7 NX_P61626 LYSC_HUMAN 4069 105200; 153450 ENSG00000090382
P68363 Tubulin alpha-1B chain 7 NX_P68363 TBA1B_HUMAN 10376 602530 ENSG00000123416
P01009 Alpha-1-antitrypsin;Short peptide from AAT 6 NX_P01009 A1AT_HUMAN 5265 107400; 613490 ENSG00000197249
P07900 Heat shock protein HSP 90-alpha 6 NX_P07900 HS90A_HUMAN 3320 140571 ENSG00000080824
Q9NZH8 Interleukin-36 gamma 6 NX_Q9NZH8 IL36G_HUMAN 56300 605542 ENSG00000136688
O43707 Alpha-actinin-4;Alpha-actinin-1 6 NX_O43707 ACTN4_HUMAN 81 603278; 604638 ENSG00000130402; ENSG00000282844
O75223 Gamma-glutamylcyclotransferase 6 NX_O75223 GGCT_HUMAN 79017 137170 ENSG00000006625
P00338 L-lactate dehydrogenase A chain 6 NX_P00338 LDHA_HUMAN 3939 150000; 612933 ENSG00000134333
P07339 Cathepsin D 6 NX_P07339 CATD_HUMAN 1509 116840; 610127 ENSG00000117984
P62987 Ubiquitin-60S ribosomal protein L40 6 NX_P62987 RL40_HUMAN 7311 191321 ENSG00000221983
P10599 Thioredoxin 6 NX_P10599 THIO_HUMAN 7295 187700 ENSG00000136810
Q9UGM3 Deleted in malignant brain tumors 1 protein 6 NX_Q9UGM3 DMBT1_HUMAN 1755 137800; 601969 ENSG00000187908
Q9UI42 Carboxypeptidase A4 6 NX_Q9UI42 CBPA4_HUMAN 51200 607635 ENSG00000128510
P47929 Galectin-7 5 NX_P47929 LEG7_HUMAN 3963; 653499 600615; 617139 ENSG00000178934; ENSG00000205076; ENSG00000282902; ENSG00000283082
Q13867 Bleomycin hydrolase 5 NX_Q13867 BLMH_HUMAN 642 602403 ENSG00000108578
Q6P4A8 Phospholipase B-like 1 5 NX_Q6P4A8 PLBL1_HUMAN 79887 NA ENSG00000121316
O75369 Filamin-B 5 NX_O75369 FLNB_HUMAN 2317 108720; 108721; 112310; 150250; 272460; 603381 ENSG00000136068
P00441 Superoxide dismutase [Cu-Zn] 5 NX_P00441 SODC_HUMAN 6647 105400; 147450 ENSG00000142168
P04792 Heat shock protein beta-1 5 NX_P04792 HSPB1_HUMAN 3315 602195; 606595; 608634 ENSG00000106211
P11142 Heat shock cognate 71 kDa protein 5 NX_P11142 HSP7C_HUMAN 3312 600816 ENSG00000109971
P58107 Epiplakin 5 NX_P58107 EPIPL_HUMAN 83481 607553 NA
P60842 Eukaryotic initiation factor 4A-I 5 NX_P60842 IF4A1_HUMAN 1973 602641 ENSG00000161960
P62937 Peptidyl-prolyl cis-trans isomerase A 5 NX_P62937 PPIA_HUMAN 5478 123840 ENSG00000196262
P63104 14-3-3 protein zeta/delta 5 NX_P63104 1433Z_HUMAN 7534 601288 ENSG00000164924
Q92820 Gamma-glutamyl hydrolase 5 NX_Q92820 GGH_HUMAN 8836 601509 ENSG00000137563
O75342 Arachidonate 12-lipoxygenase, 12R-type 4 NX_O75342 LX12B_HUMAN 242 242100; 603741 ENSG00000179477
P09211 Glutathione S-transferase P 4 NX_P09211 GSTP1_HUMAN 2950 134660 ENSG00000084207
P31025 Lipocalin-1 4 NX_P31025 LCN1_HUMAN 3933 151675 ENSG00000160349
P48594 Serpin B4 4 NX_P48594 SPB4_HUMAN 6318 600518 ENSG00000206073
Q14574 Desmocollin-3 4 NX_Q14574 DSC3_HUMAN 1825 600271; 613102 ENSG00000134762
Q5T750 Skin-specific protein 32 4 NX_Q5T750 XP32_HUMAN 100129271 NA ENSG00000198854
Q6UWP8 Suprabasin 4 NX_Q6UWP8 SBSN_HUMAN 374897 609969 ENSG00000189001
O60911 Cathepsin L2 4 NX_O60911 CATL2_HUMAN 1515 603308 ENSG00000136943
P00558 Phosphoglycerate kinase 1 4 NX_P00558 PGK1_HUMAN 5230 300653; 311800 ENSG00000102144
P04075 Fructose-bisphosphate aldolase A 4 NX_P04075 ALDOA_HUMAN 226 103850; 611881 ENSG00000149925
P07384 Calpain-1 catalytic subunit 4 NX_P07384 CAN1_HUMAN 823 114220; 616907 ENSG00000014216
P0CG05 Ig lambda-2 chain C regions 4 NA NA NA NA NA
P18206 Vinculin 4 NX_P18206 VINC_HUMAN 7414 193065; 611407; 613255 ENSG00000035403
P62258 14-3-3 protein epsilon 4 NX_P62258 1433E_HUMAN 7531 605066 ENSG00000108953; ENSG00000274474
P68871 Hemoglobin subunit beta 4 NX_P68871 HBB_HUMAN 3043 140700; 141900; 603902; 603903; 611162; 613985 ENSG00000244734
Q9C075 Keratin, type I cytoskeletal 23 4 NX_Q9C075 K1C23_HUMAN 25984 606194 ENSG00000108244; ENSG00000263309
A8K2U0 Alpha-2-macroglobulin-like protein 1 3 NX_A8K2U0 A2ML1_HUMAN 144568 610627 ENSG00000166535
P00738 Haptoglobin 3 NX_P00738 HPT_HUMAN 3240 140100; 614081 ENSG00000257017
P01011 Alpha-1-antichymotrypsin 3 NX_P01011 AACT_HUMAN 12 107280 ENSG00000196136
P02763 Alpha-1-acid glycoprotein 1 3 NX_P02763 A1AG1_HUMAN 5004 138600 ENSG00000229314
P18510 Interleukin-1 receptor antagonist protein 3 NX_P18510 IL1RA_HUMAN 3557 147679; 612628; 612852 ENSG00000136689
P22528 Cornifin-B 3 NX_P22528 SPR1B_HUMAN 6699 182266 ENSG00000169469
P30740 Leukocyte elastase inhibitor 3 NX_P30740 ILEU_HUMAN 1992 130135 ENSG00000021355
P80188 Neutrophil gelatinase-associated lipocalin 3 NX_P80188 NGAL_HUMAN 3934 600181 ENSG00000148346
Q15828 Cystatin-M 3 NX_Q15828 CYTM_HUMAN 1474 601891 ENSG00000175315
Q9HCY8 Protein S100-A14 3 NX_Q9HCY8 S10AE_HUMAN 57402 607986 ENSG00000189334
P01623 Ig kappa chain V-III region 3 NA NA NA NA NA
P01877 Ig alpha-2 chain C region 3 NX_P01877 IGHA2_HUMAN NA 147000 ENSG00000211890
P06396 Gelsolin 3 NX_P06396 GELS_HUMAN 2934 105120; 137350 ENSG00000148180
P14735 Insulin-degrading enzyme 3 NX_P14735 IDE_HUMAN 3416 146680 ENSG00000119912
P20933 N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase 3 NX_P20933 ASPG_HUMAN 175 208400; 613228 ENSG00000038002
P25788 Proteasome subunit alpha type-3 3 NX_P25788 PSA3_HUMAN 5684 176843; 176845 ENSG00000100567
P26641 Elongation factor 1-gamma 3 NX_P26641 EF1G_HUMAN 1937 130593 ENSG00000254772
P36952 Serpin B5 3 NX_P36952 SPB5_HUMAN 5268 154790 ENSG00000206075
P40926 Malate dehydrogenase, mitochondrial 3 NX_P40926 MDHM_HUMAN 4191 154100; 617339 ENSG00000146701
Q9Y6R7 IgGFc-binding protein 3 NX_Q9Y6R7 FCGBP_HUMAN 8857 617553 ENSG00000281123
O95274 Ly6/PLAUR domain-containing protein 3 2 NX_O95274 LYPD3_HUMAN 27076 609484 ENSG00000124466
P00491 Purine nucleoside phosphorylase 2 NX_P00491 PNPH_HUMAN 4860 164050; 613179 ENSG00000198805
P04080 Cystatin-B 2 NX_P04080 CYTB_HUMAN 1476 254800; 601145 ENSG00000160213
P09972 Fructose-bisphosphate aldolase C 2 NX_P09972 ALDOC_HUMAN 230 103870 ENSG00000109107
P19012 Keratin, type I cytoskeletal 15 2 NX_P19012 K1C15_HUMAN 3866 148030 ENSG00000171346
P20930 Filaggrin 2 NX_P20930 FILA_HUMAN 2312 135940; 146700; 605803 ENSG00000143631
Q96FX8 p53 apoptosis effector related to PMP-22 2 NX_Q96FX8 PERP_HUMAN 64065 609301 ENSG00000112378
Q9UIV8 Serpin B13 2 NX_Q9UIV8 SPB13_HUMAN 5275 604445 ENSG00000197641
P01625 Ig kappa chain V-IV region Len 2 NA NA NA NA NA
P01765 Ig heavy chain V-III region TIL 2 NA NA NA NA NA
P01766 Ig heavy chain V-III region BRO 2 NX_P01766 HV313_HUMAN NA NA ENSG00000211942; ENSG00000282286
P01860 Ig gamma-3 chain C region 2 NX_P01860 IGHG3_HUMAN NA 147120 NA
P01871 Ig mu chain C region 2 NX_P01871 IGHM_HUMAN NA 147020; 601495 ENSG00000211899; ENSG00000282657
P05090 Apolipoprotein D 2 NX_P05090 APOD_HUMAN 347 107740 ENSG00000189058
P06870 Kallikrein-1 2 NX_P06870 KLK1_HUMAN 3816 147910; 615953 ENSG00000167748
P07858 Cathepsin B 2 NX_P07858 CATB_HUMAN 1508 116810 ENSG00000164733
P08865 40S ribosomal protein SA 2 NX_P08865 RSSA_HUMAN 3921 150370; 271400 ENSG00000168028
P11279 Lysosome-associated membrane glycoprotein 1 2 NX_P11279 LAMP1_HUMAN 3916 153330 ENSG00000185896
P13473 Lysosome-associated membrane glycoprotein 2 2 NX_P13473 LAMP2_HUMAN 3920 300257; 309060 ENSG00000005893
P19971 Thymidine phosphorylase 2 NX_P19971 TYPH_HUMAN 1890 131222; 603041 ENSG00000025708
P23284 Peptidyl-prolyl cis-trans isomerase B 2 NX_P23284 PPIB_HUMAN 5479 123841; 259440 ENSG00000166794
P23396 40S ribosomal protein S3 2 NX_P23396 RS3_HUMAN 6188 600454 ENSG00000149273
P25705 ATP synthase subunit alpha, mitochondrial 2 NX_P25705 ATPA_HUMAN 498 164360; 615228; 616045 ENSG00000152234
P27482 Calmodulin-like protein 3 2 NX_P27482 CALL3_HUMAN 810 114184 ENSG00000178363
P31949 Protein S100-A11 2 NX_P31949 S10AB_HUMAN 6282 603114 ENSG00000163191
P40121 Macrophage-capping protein 2 NX_P40121 CAPG_HUMAN 822 153615 ENSG00000042493
P42357 Histidine ammonia-lyase 2 NX_P42357 HUTH_HUMAN 3034 235800; 609457 ENSG00000084110
P47756 F-actin-capping protein subunit beta 2 NX_P47756 CAPZB_HUMAN 832 601572 ENSG00000077549
P48637 Glutathione synthetase 2 NX_P48637 GSHB_HUMAN 2937 231900; 266130; 601002 ENSG00000100983
P49720 Proteasome subunit beta type-3 2 NX_P49720 PSB3_HUMAN 5691 602176 ENSG00000277791; ENSG00000275903
P50395 Rab GDP dissociation inhibitor beta 2 NX_P50395 GDIB_HUMAN 2665 600767 ENSG00000057608
P59998 Actin-related protein 2/3 complex subunit 4 2 NX_P59998 ARPC4_HUMAN 10093 604226 ENSG00000241553
P61160 Actin-related protein 2 2 NX_P61160 ARP2_HUMAN 10097 604221 ENSG00000138071
P61916 Epididymal secretory protein E1 2 NX_P61916 NPC2_HUMAN 10577 601015; 607625 ENSG00000119655
P04745 Alpha-amylase 1 23 NX_P04745 AMY1_HUMAN 276; 277; 278 104700; 104701; 104702 ENSG00000174876; ENSG00000187733; ENSG00000237763
Q9NZT1 Calmodulin-like protein 5 8 NX_Q9NZT1 CALL5_HUMAN 51806 605183 ENSG00000178372
P12273 Prolactin-inducible protein 6 NX_P12273 PIP_HUMAN 5304 176720 ENSG00000159763
Q96DA0 Zymogen granule protein 16 homolog B 5 NX_Q96DA0 ZG16B_HUMAN 124220 NA ENSG00000162078; ENSG00000283056
P01036 Cystatin-S 5 NX_P01036 CYTS_HUMAN 1472 123857 ENSG00000101441
Q8TAX7 Mucin-7 2 NX_Q8TAX7 MUC7_HUMAN 4589 158375; 600807 ENSG00000171195
P01037 Cystatin-SN 2 NX_P01037 CYTN_HUMAN 1469 123855 ENSG00000170373
P09228 Cystatin-SA 2 NX_P09228 CYTT_HUMAN 1470 123856 ENSG00000170369
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