This is a command line version of qPCR normalization software qBase (now proprietary). This program transforms raw expression measurements into calibrated relative quantities using a modified delta-delta-Ct method accounting for primer efficiency. The qBase alogrithm can use several normalizer genes and allows for inter-plates calibration. Original publication describing the method: Hellemans, J. et al. (2007) qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data. Genome Biol. 8(2):R19.
This script was used in the following manuscripts:
- Fedrigo O., A.D. Pfefferle, C.C. Babbitt, R. Haygood, C.E. Wall, and G.A. Wray. (2011) Multiple lines of evidence link glucose transporters to human brain evolution. Brain, Behavior and Evolution. 78:315–326.
- Pfefferle A.D., L.R. Warner, C.W. Wang, W. J. Nielsen, C.C. Babbitt, O. Fedrigo, and G.A. Wray. (2011) Comparative expression analysis of the phosphocreatine circuit in extant primates: Implications for human brain evolution. Journal of Human Evolution. 60(2):205-12.
- Cruz-Gordillo P., O. Fedrigo, G.A. Wray, and C.C. Babbitt. (2010) Extensive Changes in the Expression of the Opioid Genes between Humans and Chimpanzees. Brain, Behavior and Evolution.76:154-162.