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@@ -2,4 +2,4 @@ channels: | |
- conda-forge | ||
- bioconda | ||
dependencies: | ||
- bioconda::genmod=3.8.2 | ||
- bioconda::genmod=3.8.3 |
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@@ -2,4 +2,4 @@ channels: | |
- conda-forge | ||
- bioconda | ||
dependencies: | ||
- bioconda::genmod=3.8.2 | ||
- bioconda::genmod=3.8.3 |
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|
@@ -2,4 +2,4 @@ channels: | |
- conda-forge | ||
- bioconda | ||
dependencies: | ||
- bioconda::genmod=3.8.2 | ||
- bioconda::genmod=3.8.3 |
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|
@@ -2,4 +2,4 @@ channels: | |
- conda-forge | ||
- bioconda | ||
dependencies: | ||
- bioconda::genmod=3.8.2 | ||
- bioconda::genmod=3.8.3 |
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channels: | ||
- conda-forge | ||
- bioconda | ||
dependencies: | ||
- bioconda::motus=3.1.0 |
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process MOTUS_PREPLONG { | ||
tag "$meta.id" | ||
label 'process_single' | ||
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conda "${moduleDir}/environment.yml" | ||
container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? | ||
'https://depot.galaxyproject.org/singularity/motus:3.1.0--pyhdfd78af_0': | ||
'biocontainers/motus:3.1.0--pyhdfd78af_0' }" | ||
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input: | ||
tuple val(meta), path(reads) | ||
path db | ||
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output: | ||
tuple val(meta), path("*.gz"), emit: out | ||
path "versions.yml" , emit: versions | ||
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when: | ||
task.ext.when == null || task.ext.when | ||
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script: | ||
def args = task.ext.args ?: '' | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
def refdb = db ? "-db ${db}" : "" | ||
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""" | ||
motus \\ | ||
prep_long \\ | ||
$args \\ | ||
-i $reads \\ | ||
$refdb \\ | ||
-t $task.cpus \\ | ||
-o ${prefix}.gz \\ | ||
2> >(tee ${prefix}.log >&2) | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
motus: \$(motus --version 2>&1 | sed 's/.* \\([0-9]*\\.[0-9]*\\.[0-9]*\\) .*/\\1/') | ||
END_VERSIONS | ||
""" | ||
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stub: | ||
def args = task.ext.args ?: '' | ||
def prefix = task.ext.prefix ?: "${meta.id}" | ||
""" | ||
echo '' | gzip > ${prefix}.gz | ||
cat <<-END_VERSIONS > versions.yml | ||
"${task.process}": | ||
motus: \$(motus --version 2>&1 | sed 's/.* \\([0-9]*\\.[0-9]*\\.[0-9]*\\) .*/\\1/') | ||
END_VERSIONS | ||
""" | ||
} |
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name: "motus_preplong" | ||
description: Taxonomic meta-omics profiling using universal marker genes | ||
keywords: | ||
- classify | ||
- metagenomics | ||
- fastq | ||
- taxonomic profiling | ||
tools: | ||
- "motus": | ||
description: "Marker gene-based operational taxonomic unit (mOTU) profiling" | ||
homepage: "https://motu-tool.org/" | ||
documentation: "https://github.com/motu-tool/mOTUs/wiki" | ||
tool_dev_url: "https://github.com/motu-tool/mOTUs" | ||
doi: "10.1186/s40168-022-01410-z" | ||
licence: ["GPL v3"] | ||
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input: | ||
- - meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. `[ id:'sample1', single_end:false ]` | ||
- reads: | ||
type: file | ||
description: Long read file to convert, can be fasta(.gz) or fastq(.gz) | ||
pattern: "*.{gz}" | ||
- - db: | ||
type: directory | ||
description: | | ||
mOTUs database downloaded by `motus downloadDB` | ||
pattern: "db_mOTU/" | ||
output: | ||
- out: | ||
- meta: | ||
type: map | ||
description: | | ||
Groovy Map containing sample information | ||
e.g. `[ id:'sample1', single_end:false ]` | ||
- "*.gz": | ||
type: file | ||
description: converted file (gzipped), ready to be used by motus profile | ||
pattern: "*.gz" | ||
- versions: | ||
- versions.yml: | ||
type: file | ||
description: File containing software versions | ||
pattern: "versions.yml" | ||
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authors: | ||
- "@sofstam" | ||
maintainers: | ||
- "@sofstam" |
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nextflow_process { | ||
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name "Test Process MOTUS_PREPLONG" | ||
script "../main.nf" | ||
process "MOTUS_PREPLONG" | ||
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tag "modules" | ||
tag "modules_nfcore" | ||
tag "motus" | ||
tag "motus/downloaddb" | ||
tag "motus/preplong" | ||
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setup { | ||
run("MOTUS_DOWNLOADDB") { | ||
script "modules/nf-core/motus/downloaddb/main.nf" | ||
process { | ||
""" | ||
input[0] = Channel.of([ | ||
file('https://raw.githubusercontent.com/motu-tool/mOTUs/master/motus/downloadDB.py') | ||
]) | ||
""" | ||
} | ||
} | ||
} | ||
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test("sarscov2 - fasta") { | ||
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when { | ||
process { | ||
""" | ||
input[0] = [ | ||
[ id:'test', single_end:false ], // meta map | ||
file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test_2.fastq.gz', checkIfExists: true) | ||
] | ||
input[1] = MOTUS_DOWNLOADDB.out.db | ||
""" | ||
} | ||
} | ||
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then { | ||
assertAll( | ||
{ assert process.success }, | ||
{ assert snapshot(process.out).match() } | ||
) | ||
} | ||
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} | ||
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test("sarscov2 - fasta - stub") { | ||
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options "-stub" | ||
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when { | ||
process { | ||
""" | ||
input[0] = [ | ||
[ id:'test', single_end:false ], // meta map | ||
file(params.modules_testdata_base_path + 'genomics/sarscov2/nanopore/fastq/test_2.fastq.gz', checkIfExists: true) | ||
] | ||
input[1] = MOTUS_DOWNLOADDB.out.db | ||
""" | ||
} | ||
} | ||
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then { | ||
assertAll( | ||
{ assert process.success }, | ||
{ assert snapshot(process.out).match() } | ||
) | ||
} | ||
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|
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} | ||
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} |
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