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[WIP] Improve off-reference coordinate and rGFA support #3891
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The overall goal here is to allow calling within large insertions: With the HPRC human pangenomes, we presently project to hg38 using
vg surject
for Giraffe/DeepVariant or vg's VCF projection fordeconstruct
andcall
. In all cases, this makes looking at variants within insertions difficult: they could be lost insurject
or get buried in giant nested alleles in VCF.As far as the vg tools are concerned, we could support variants within insertions provided they get unique "reference' paths to project on. rGFA is what minigraph uses to define these paths: each node in the graph gets a "stable" coordinate on one of the input haplotypes. This is effectively a path cover of the graph that gives each position a unique coordinate.
There are some challenges with incorporating this into vg:
As of right now, this PR adds an option to
vg paths
to compute the rGFA cover using a greedy algorithm, allowing specification of a minimum fragment size. This is nearly enough to start playing around with some applications. But there's still work to be done on the metadata questions above before it can be merged.