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Ctf_estimate_from_psd_v31

DavidHerreros edited this page Sep 6, 2019 · 2 revisions

!xmipp_ctf_estimate_from_psd (v3.1)

Usage

Adjust a parametric model to a PSD file. The PSD is enhanced (See article). And finally, the CTF is fitted to the PSD, being guided by the enhanced PSD (See article).

See also Ctf_estimate_from_micrograph_v3 Ctf_enhance_psd_v3

Parameters

$``: PSD file

Downsampling $--downSamplingPerformed <F1>

CTF fit: Optimization constraints $--min_freq &lt;fmin0.03> $--max_freq &lt;fmax0.35> $--fastDefocus &lt;lambda2> <size=10> $--defocus_range &lt;D8000> $: Show optimization process $: By default, noise is astigmatic $--enhance_weight &lt;w1> $--model_simplification &lt;s0> $--bootstrapFit &lt;N-1>

CTF fit: Output CTF models $--ctfmodelSize &lt;size256>

PSD enhancement $: Bandpass cutoff. Normalized to 0.5 If fmax>0.35, f1 default=0.01 If fmax<0.35, f1 default=0.02 $: Bandpass cutoff. Normalized to 0.5. If fmax>0.35, f2 default=0.08 If fmax<0.35, f2 default=0.15

CTF description $: ctfparam file Parameters from this file are overriden by the parameters in the command line $: Angstroms/pixel. Ex: 1.4 This parameter is compulsory if a CTF is needed. $: Accelerating voltage (kV). Ex: 200 This parameter is compulsory if a CTF is needed. $: Milimiters. Ex: 5.6 This parameter is compulsory if a CTF is needed. $: Defocus in Angstroms (Ex: 2000) $: If astigmatic $--azimuthal_angle &lt;ang0> $--chromatic_aberration &lt;Ca0> $--energy_loss &lt;espr0> $--lens_stability &lt;ispr0> $--convergence_cone &lt;alpha0> $--longitudinal_displace &lt;DeltaF0> $--transversal_displace &lt;DeltaR0> $--Q0 &lt;Q00> $--K &lt;K1>

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