Applications Utilizing CRISPR Cas9

Citation: Toh, BCY, Ling, MHT. 2023. Applications Utilizing CRISPR/Cas9. Novel Research in Sciences 14(1):NRS.000826.

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Clustered regularly interspaced short palindromic repeats (CRISPR) Cas (CRISPR-associated) system is an adaptive immune system used by prokaryotes, which has been adapted for many laboratory applications. Here, we illustrate applications of CRISPR/Cas9 in 7 areas: (i) genome engineering, (ii) edition of single-stranded RNA (ssRNA), (iii) high throughput gene screening, (iv) creating disease models, (v) live labelling of chromosomal loci, (vi) epigenome editing, and (vii) regulation of endogenous gene expression.