Signal Peptidase Complex Subunit 1 (SPCS1) and Hydroxyacyl CoA Dehydrogenase Beta Subunit (HADHB) are Suitable Reference Genes in Human Lungs.

Citation: Too, IHK, Ling, MHT. 2012. Signal Peptidase Complex Subunit 1 (SPCS1) and Hydroxyacyl-CoA Dehydrogenase Beta Subunit (HADHB) are Suitable Reference Genes in Human Lungs. ISRN Bioinformatics 2012, Article ID 790452.

Link to [Full Text] and [PDF].

Here is a permanent link to this [PDF] in my own archive.

Lung cancer is a common cancer and expression profiling can provide an accurate indication to advance the medical intervention. However, this requires the availability of stably expressed genes as reference. Recent studies had shown that genes that are stably expressed in a tissue may not be stably expressed in other tissues suggesting the need to identify stably expressed genes in each tissue for use as reference genes. DNA microarray analysis has been used to identify those reference genes with low fluctuation. Fourteen datasets with different lung conditions were employed in our study. Coefficient of variance, followed by NormFinder, was used to identify stably expressed genes. Our results showed that classical reference genes such as GAPDH and HPRT1 were highly variable; thus, unsuitable as reference genes. SPCS1 and HADHB, involving in fundamental biochemical processes, demonstrated high expression stability suggesting that their suitability in human lung cell profiling.